ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI

Agustina Setiawati, Sri Hartati Yuliani, Enade Perdana Istyastono, Michael Raharja Gani, Evy Fenny Veronica, Dina Christin Ayuning Putri, Reza Eka Putra, David Chandra Putra, Agnes Mutiara Kurniawan

Abstract


Rapid and simple quantitative analysis isoflavones tempe using densitometric TLChas been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5:0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distanceof 1 ?L sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linearrelationship obtained at 0.08 - 2 ?g/spot with r= 0.9986. The LOD and LOQ of isoflavone were0.014 ?g/spot and 0.048 ?g/spot. Genistein contained in tempe was 0.151 0.005 % b/b.

Keywords


tempe; isoflavon; TLC

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DOI: https://doi.org/10.24071/jpsc.0080

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Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community)

Published by Faculty of Pharmacy, Universitas Sanata Dharma Yogyakarta

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