ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI

Agustina Setiawati, Sri Hartati Yuliani, Enade Perdana Istyastono, Michael Rahardja Gani, Evy Fenny Veronica, Dina Christin Ayuning Putri, Reza Eka Putra, David Chandra Putra, Agnes Mutiara Kurniawan

Abstract


Abstract: Rapid and simple quantitative analysis isoflavones tempe using densitometric TLChas been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5:0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distanceof 1 ?L sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linearrelationship obtained at 0.08 - 2 ?g/spot with r= 0.9986. The LOD and LOQ of isoflavone were0.014 ?g/spot and 0.048 ?g/spot. Genistein contained in tempe was 0.151 0.005 % b/b.


Keywords: tempe, isoflavon, TLC


Full Text:

PDF


DOI: https://doi.org/10.24071/jpsc.0080

Refbacks

  • There are currently no refbacks.


Copyright (c) 1970 Journal of Pharmaceutical Sciences and Community

 

 

 

 

 

 

 

  

Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community)

Published by Faculty of Pharmacy, Universitas Sanata Dharma Yogyakarta

Creative Commons Licence
This work is licensed under a Creative Commons Attribution 4.0 International License.

Web Analytics JPSC Stats