Optimization of Naringenin Self-Nano Emulsifying Drug Delivery System (SNEDDS) Formula with D-optimal Mixture Design Method

Gendis Purno Yudanti(1*), Ilham Kuncahyo(2), Endang Diyah Ikasari(3),

(1) Stikes Cendekia Utama Kudus
(2) Faculty of Pharmacy, University Setiabudi Surakarta
(3) Faculty of Pharmacy, Sekolah Tinggi Farmasi Yayasan Sekolah Tinggi Ilmu Farmasi, Semarang
(*) Corresponding Author

Abstract


This study aimed to optimize and formulate the poorly water-soluble naringenin in the Self-Nano Emulsifying Drug Delivery System (SNEDDS) using D-optimal mixture design. D-optimal mixture design was used to optimize SNEDDS loading naringenin by selecting SNEDDS composition as an independent factor and SNEDDS characterization as a response. SNEDDS in the optimal formula were characterized, including transmittance, particle size, emulsification time, and drug loading. Triacetin, Tween 80, and transcutol p were respectively the selected oil, surfactant, and co-surfactant phases for their greatest ability to dissolve naringenin. The optimization results showed that the optimal formula was that using 10% triacetin, 70% of Tween 80, and 20% of transcutol p. SNEDDS loading naringenin produced nanoemulsion with 88.74±2.27 % of transmittance, 14.8 nm of particle size, 51.13 ± 4.53 mg/L of drug loading, and 18.58 ± 0.62 second of emulsification time. This study concludes that the D-optimal mixture design can be used to optimize and prepare the SNEDDS loading poorly-water soluble naringenin.  


Keywords


Naringenin; SNEDDS; Triacetin; Transcutol p; Tween 80

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formula shows the optimum area which is shown in the grey area. This overlay plot is a combination of contour plots on four critical parameters, namely emulsification time, drug loading, the transmittance percentage and particle size. The red dot in one of the optimum areas is the optimum formula suggested by the Design Expert results from several predictions of the optimum formula in the optimum area, which can be seen in Figure 4. CONCLUSIONS Based on the research that was conducted, the following conclusions can be drawn: The optimal proportions of triacetin, tween 80 and transcutol P components in the manufacture of naringenin SNEDDS using the D – optimal mixture design method obtained a ratio of triacetin, tween 80, and transcutol P of 10.16 : 69.84 : 20. 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DOI: https://doi.org/10.24071/jpsc.004319

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