EFFECTS OF PARTICLE SIZE, EXTRACTION TIME, AND SOLVENT  ON DAIDZEIN YIELD EXTRACTED FROM TEMPEH

Sri Hartati Yuliani; Petrus Damiani Tosan Aji; Arini Safti Sandrapitaloka; Fransiska Revana Restiana; Michael Raharja Gani; Florentinus Dika Octa Riswanto

doi:10.24071/jpsc.001794

EFFECTS OF PARTICLE SIZE, EXTRACTION TIME, AND SOLVENT ON DAIDZEIN YIELD EXTRACTED FROM TEMPEH

Sri Hartati Yuliani^(1*), Petrus Damiani Tosan Aji⁽²⁾, Arini Safti Sandrapitaloka⁽³⁾, Fransiska Revana Restiana⁽⁴⁾, Michael Raharja Gani⁽⁵⁾, Florentinus Dika Octa Riswanto⁽⁶⁾,

(1) Faculty of Pharmacy, Sanata Dharma University
(2) Faculty of Pharmacy, Sanata Dharma University
(3) Faculty of Pharmacy, Sanata Dharma University
(4) Faculty of Pharmacy, Sanata Dharma University
(5) Faculty of Pharmacy, Sanata Dharma University
(6) Faculty of Pharmacy, Sanata Dharma University
(*) Corresponding Author

Abstract

Daidzein, one of the isoflavone aglycones contained in tempeh, has several biological activities such as anti-inflammatory, anti-oxidant, anti-breast cancer, and suppression of expression of matrix metalloprotease-9. As a fermented product from soybeans, daidzein content in tempeh was found in higher concentration compared to the soybean raw material. It was important to optimize several factors affecting extraction process such as particle size of tempeh simplicia, extraction time, and solvent in order to develop an effective method for the daidzein isolation from the tempeh or other natural products. Evaluation of extraction factors was conducted by applying variations for each factor followed by quantitative analysis using HPLC methods. The optimization condition was performed by daidzein standard and achieved with the particle size of tempeh simplicia of 1.2 mm, extraction time of 360 minutes, and 70% ethanol was used as solvent. Furthermore, the optimized condition was applied for the daidzein isolation from tempeh, a soybean fermented product.

Keywords

daidzein; extraction time; particle size; solvent; soybean fermented product

Full Text:

PDF

References

Hernndez, Y., Lobo, M.G., and Gonzlez, M., 2009. Factors affecting sample extraction in the liquid chromatographic determination of organic acids in papaya and pineapple. Food Chem., 114, 734741.

Hong, G.-E., Mandal, P.K., Lim, K.-W., and Lee, C.-H., 2012. Fermentation increases isoflavone aglycone in black soybean pulp. Asian J. Anim. and Vet. Adv., 7(6), 502511.

Jyoti, Agrawal, S.S., Saxena, S., and Sharma, A., 2015. Phytoestrogen Genistein: Its extraction and isolation from soybean seeds. Int. J. Pharm. and Phyto. Res., 7(6), 11211126.

Kuligowski, M., Paw?owska, K., Jasi?ska-Kuligowska, I., and Nowak, J., 2016. Iso?avone composition, polyphenols content and antioxidative activity of soybean seeds during tempeh fermentation. CyTA - Journal of Food, 15 (1), 17.

Lakshmi, M.C., Rao, L.J., Ravi, R., Raghavarao, K.S.M.S., Rao, L.J., Ravi, R., Raghavarao, K.S.M.S., Lakshmi, M.C., Rao, L.J., Ravi, R., and Raghavarao, K.S.M.S., 2013. Extraction and concentration of isoflavones from soybean (Glycine max). Sep. Sci. and Techn., 48, 166174.

List, P.H. and Schmidt, P.C., 1989, Phytopharmaceutical Technology, CRC Press, North America, 101-110

Liu, X., Suzuki, N., Laxmi, Y.R.S., Okamoto, Y., and Shibutani, S., 2012. Anti-breast cancer potential of daidzein in rodents. Life Sciences, 91 (1112), 415419.

Oh, H.J., Kang, Y.G., Na, T.Y., Kim, H.J., Park, J.S., Cho, W.J., and Lee, M.O., 2013. Identification of daidzein as a ligand of retinoic acid receptor that suppresses expression of matrix metalloproteinase-9 in HaCaT cells. Mol. and Cell. Endo., 376 (12), 107113.

Park, M.-H., Ju, J.-W., Park, M.-J., and Han, J.S., 2013. Daidzein inhibits carbohydrate digestive enzymes in vitro and alleviates postprandial hyperglycemia in diabetic mice. Eur. J. Pharm., 712, 4852.

Peng, Y., Shi, Y., Zhang, H., Mine, Y., and Tsao, R., 2017. Anti-inflammatory and anti-oxidative activities of daidzein and its sulfonic acid ester derivatives. Journal of Functional Foods, 35, 635640.

Rostagno, M.A., Palma, M., and Barroso, C.G., 2003. Ultrasound-assisted extraction of soy isoflavone. J. Chrom. A, 1012(2), 119128.

Rostagno, M.A., Villares, A., Guillamn, E., Garca-Lafuente, A., and Martnez, J.A., 2009. Sample preparation for the analysis of isoflavones from soybeans and soy foods. J. Chrom. A, 1216(1), 229.

Sapri, Fitriani, A., and Narulita, R., 2014. Pengaruh Ukuran Serbuk Simplisia Terhadap Rendemen Ekstrak Etanol Daun Sirsak (Annona Muricata L .) Dengan Metode Maserasi. In: Prosiding Seminar Nasional Kimia. 14.

Takaoka, O., Mori, T., Ito, F., Okimura, H., Kataoka, H., Tanaka, Y., Koshiba, A., Kusuki, I., Shigehiro, S., Amami, T., and Kitawaki, J., 2018. Daidzein-rich isoflavone aglycones inhibit cell growth and inflammation in endometriosis. The Journal of Steroid Biochemistry and Molecular Biology, 181, 125132.

Yang, G., Huang, Y., Nan, G., Chen, H., Zeng, A., and Bian, X., 2013. Solubility of daidzein in the binary system of ethanol and water. Journal of Molecular Liquids, 180, 160163.

Yoshiara, L.Y., Madeira, T.B., Delaroza, F., Bonifcio, J., and Ida, E.I., 2012. Optimization of soy isoflavone extraction with different solvents using the simplex-centroid mixture design. International Journal of Food Sciences and Nutrition, 63(8), 978986.

Yuliani, S.H., Gani, M.R., Istyastono, E.P., and Riswanto, F.D.O., 2018. Optimization of genistein and daidzein extraction from a tempeh-fermented product of soybean. J. Pharm. and. Pharmacog. Res., 6 (4), 231241.

Yuliani, S.H., Istyastono, E.P., and Riswanto, F.D.O., 2016. The cytotoxic activity on T47D breast cancer cell of Genistein-standardized ethanolic extract of tempeh - a fermented product of soybean (Glycine max). Orient. J. Chem., 32(3), 16191624.

Zhang, Q.W., Lin, L.G., Ye, W.C., 2018. Techniques for Extraction and Isolation of Natural Products - a Comprehensive Review. Chinese Medicine, 13, 2045.

Zhang, E.J., Ng, K.M., and Luo, K.Q., 2007. Extraction and purification of isoflavones from soybeans and characterization of their estrogenic activities. J. Agricul. and Food Chem., 55 (17), 69406950.

Zhu, X.Y., Lin, H.M., Xie, J., Chen, S.S., and Wang, P., 2011. Homogenate extraction of isoflavones from soybean meal by orthogonal design. Journal of Scientific and Industial Research, 70, 455460.

DOI: https://doi.org/10.24071/jpsc.001794