ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI

Agustina Setiawati, Sri Hartati Yuliani, Enade Perdana Istyastono, Michael Rahardja Gani, Evy Fenny Veronica, Dina Christin Ayuning Putri, Reza Eka Putra, David Chandra Putra, Agnes Mutiara Kurniawan

Abstract


Abstract: Rapid and simple quantitative analysis isoflavones tempe using densitometric TLC has been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5: 0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distance of 1 μL sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linear relationship obtained at 0.08 - 2 μg/spot with r= 0.9986. The LOD and LOQ of isoflavone were 0.014 μg/spot and 0.048 μg/spot. Genistein contained in tempe was 0.151± 0.005 % b/b.


Keywords: tempe, isoflavon, TLC


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DOI: http://dx.doi.org/10.24071/jpsc.11180

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Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community)

Published by Faculty of Pharmacy, Universitas Sanata Dharma Yogyakarta

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