Optimization and Physical Characterization of Kojic Acid Nanoemulsion Formula As A Whitening Agent

Yovi Guanse; Rini Dwiastuti; Brigita Tuelvin Jenita Gandi; Lutfi Chabib

doi:10.24071/jpsc.009968

Optimization and Physical Characterization of Kojic Acid Nanoemulsion Formula As A Whitening Agent

Yovi Guanse⁽¹⁾, Rini Dwiastuti^(2*), Brigita Tuelvin Jenita Gandi⁽³⁾, Lutfi Chabib⁽⁴⁾,

(1) Universitas Sanata Dharma
(2) Universitas Sanata Dharma
(3) Universitas Sanata Dharma
(4) Universitas Islam Indonesia
(*) Corresponding Author

Abstract

Kojic acid (KA) as a topical formulation prevents hyperpigmentation through the inhibition of tyrosinase activity. However, KA has higher solubility in nonpolar solvents. Nanoparticle technology can be a novel innovation to enhance KA absorption into the skin. This study aims to improve the delivery of topically applied KA by enhancing absorption into the stratum corneum by optimizing the concentration of surfactant Tween 80 and co-surfactant Span 80. The nanoemulsion uses KA as an active ingredient and virgin coconut oil as the oil phase. The optimum area was analyzed using Design-Expert® 13 software, with a pure experimental design based on a 2x2 factorial design. The results indicate that all formulas are within the optimal region. Therefore, the determination of the optimal point can be grounded on the smallest composition within the optimal region with the objective of maximizing material efficiency, which is a formula with a concentration of Tween 80 and Span 80 of 10 grams and 3 grams, respectively. The particles are a spherical smooth shape and demonstrate an efficacy of 94.117% in inhibiting tyrosinase activity.

Keywords

Kojic acid; Nanoemulsion; Span 80; Tween 80; VCO

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References

Alhasso, B., Ghori, M.U., Conway, B.R., 2023. Development of nanoemulsions for topical application of mupirocin. Pharmaceutics, 15(2).

Boskabadi, M., Saeedi, M., Akbari, J., Morteza-Semnani, K., Hashemi, S.M.H., Babaei, A., 2021. Topical gel of vitamin A solid lipid nanoparticles: A hopeful promise as a dermal delivery system. Advanced Pharmaceutical Bulletin, 11(4), 663–674.

Chaudhary, J., 2014. Production technology and applications of kojic acid. Annual Research & Review in Biology, 4(21), 3165–3196.

Danaei, M., Dehghankhold, M., Ataei, S., Hasanzadeh Davarani, F., Javanmard, R., Dokhani, A., Khorasani, S., Mozafari, M.R., 2018. Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics, 10(2), 1–17.

Dolowy, M., Kozik, V., Bak, A., Jampilek, J., Barbusinski, K., Thomas, M., Pyka-Pajak, A., 2017. Rapid and simple TLC-densitometric method for assay of clobetasol propionate in topical solution. Molecules, 22(11).

El-Feky, G.S., El-Banna, S.T., El-Bahy, G.S., Abdelrazek, E.M., Kamal, M., 2017. Alginate coated chitosan nanogel for the controlled topical delivery of silver sulfadiazine. Carbohydrate Polymers, 177, 194–202.

Ermawati, D.E., Jannah, S., 2023. The effect of surfactant concentration on particle size and loading dose of immunity jamu’s ethanolic extract SNEDDS (Self -Nano Emulsifying Drugs Delivery System) Traditional Medicine Journal, 28(2), 102-111.

Furi, M., Alfatma, A., Dona, R., Fernando, A., Aryani, F., Umar, R.U., Muharni, S., Husnawati, Suhery, W.N., Octaviani, M., 2022. Uji inhibitor enzim tirosinase ekstrak dan fraksi daun kedabu (Sonneratia ovata Backer) secara in-vitro. Jurnal Ilmiah Manuntung, 8(2), 201–214.

Ghasemiyeh, P., Mohammadi-Samani, S., 2020. Potential of nanoparticles as permeation enhancers and targeted delivery options for skin: Advantages and disadvantages. Drug Design, Development and Therapy, 14, 3271–3289.

Gomes, C., Silva, A.C., Marques, A.C., Lobo, J.S., Amaral, M.H., 2020. Biotechnology applied to cosmetics and aesthetic medicines. Cosmetics, 7(2), 1–14.

Gumustas, M., Sengel-Turk, C.T., Gumustas, A., Ozkan, S.A., Uslu, B., 2017. Effect of polymer-based nanoparticles on the assay of antimicrobial drug delivery systems, Multifunctional Systems for Combined Delivery, Biosensing and Diagnostics. Elsevier Inc.

Hidayatri, N., 2021. Aktivitas penghambatan enzim tirosinase secara in vitro solid lipid nanopartikel tetrahidrokurkumin. Jurnal Sains dan Kesehatan, 3(6), 767–772.

Jusril, N.A., Abu Bakar, S.I., Khalil, K.A., Md Saad, W.M., Wen, N.K., Adenan, M.I., 2022. Development and optimization of nanoemulsion from ethanolic extract of Centella asiatica (NanoSECA) using D-Optimal Mixture design to improve blood-brain barrier permeability. Evidence-based Complementary and Alternative Medicine, 2022.

Khairati, M., 2023. Virgin coconut oil: A review. International Journal of Research and Review, 10(6), 70–75.

Lukić, M., Pantelić, I., Savić, S.D., 2021. Towards optimal ph of the skin and topical formulations: From the current state of the art to tailored products. Cosmetics, 8(3).

Ma, Z.F., Lee, Y.Y., 2016. Virgin coconut oil and its cardiovascular health benefits. Natural Product Communications, 11(8), 1151–1152.

Mohiuddin, A., 2016. Skin lightening & management of hyperpigmentation AK. International Journal for Research in Biology & Pharmacy Research, 2(3), 58–66.

Mulia, K., Ramadhan, R.M.A., Krisanti, E.A., 2018. Formulation and characterization of nanoemulgel mangosteen extract in virgin coconut oil for topical formulation. MATEC Web of Conferences, 156.

Muralidhara, B.K., Wong, M., 2020. Critical considerations in the formulation development of parenteral biologic drugs. Drug Discovery Today, 25(3), 574–581.

Németh, Z., Csóka, I., Semnani Jazani, R., Sipos, B., Haspel, H., Kozma, G., Kónya, Z., Dobó, D.G., 2022. Quality by design-driven zeta potential optimisation study of liposomes with charge imparting membrane additives. Pharmaceutics, 14(9).

Patzelt, A., Mak, W.C., Jung, S., Knorr, F., Meinke, M.C., Richter, H., Rühl, E., Cheung, K.Y., Tran, N.B.N.N., Lademann, J., 2017. Do nanoparticles have a future in dermal drug delivery? Journal of Controlled Release, 246, 174–182.

Phasha, V., Senabe, J., Ndzotoyi, P., Okole, B., Fouche, G., Chuturgoon, A., 2022. Review on the use of kojic acid: A skin-lightening ingredient. Cosmetics, 9(3), 1–11.

Ripaldo, F., 2020. Uji aktivitas inhibitor enzim tirosinase dan uji antioksidan ekstrak etanol buah harendong (Melastoma malabathricum L.) secara in vitro. Indonesia Natural Research Pharmaceutical Journal, 5(1), 1–16.

Romes, N.B., Wahab, R.A., Abdul Hamid, M., Hashim, S.E., 2022. D-optimal design-assisted Elaeis guineensis leaves extract in olive oil-sunflower seed nanoemulsions: Development, characterization, and physical stability. Journal of Dispersion Science and Technology, 43(2), 289–301.

Saeedi, M., Eslamifar, M., Khezri, K., 2019. Kojic acid applications in cosmetic and pharmaceutical preparations. Biomedicine and Pharmacotherapy, 110(September 2018), 582–593.

Shaker, D.S., Ishak, R.A.H., Ghoneim, A., Elhuoni, M.A., 2019. Nanoemulsion: A review on mechanisms for the transdermal delivery of hydrophobic and hydrophilic drugs. Scientia Pharmaceutica, 87(3).

Sopyan, I., Gozali, D., Sriwidodo, Guntina, R.K., 2022. Design-Expert Software (Doe): An application tool for optimization in pharmaceutical preparations formulation. International Journal of Applied Pharmaceutics, 14(4), 55–63.

Ströher, D.J., De Oliveira, M.F., Martinez-Oliveira, P., Pilar, B.C., Cattelan, M.D.P., Rodrigues, E., Bertolin, K., Gonçalves, P.B.D., Piccoli, J.D.C.E., Manfredini, V., 2020. Virgin coconut oil associated with high-fat diet induces metabolic dysfunctions, adipose inflammation, and hepatic lipid accumulation. Journal of Medicinal Food, 23(7), 689–698.

Sun, R., He, H., Wan, Y., Li, L., Sha, J., Jiang, G., Li, Y., Li, T., Ren, B., 2021. Kojic acid in fourteen mono-solvents: Solubility data, Hansen solubility parameter and thermodynamic properties. Journal of Chemical Thermodynamics, 152(1), 1–13.

Thango, B.A., 2022. Application of the Analysis of Variance (ANOVA) in the interpretation of power transformer faults. Energies, 15(19).

Umate, N., Kuchewar, V., Parwe, S., 2022. A narrative review on use of virgin coconut oil in dermatology. Journal of Indian System of Medicine, 10(2), 86.

Wang, Q., Wang, Y., Xie, Y., Adu-Frimpong, M., Wei, C., Yang, X., Cao, X., Deng, W., Toreniyazov, E., Ji, H., Xu, X., Yu, J., 2021. Nonionic surfactant vesicles as a novel drug delivery system for increasing the oral bioavailability of Ginsenoside Rb1. Food Bioscience, 42(June), 101064.

Wang, X.R., Cheng, H.M., Gao, X.W., Zhou, W., Li, S.J., Cao, X.L., Yan, D., 2019. Intercalation assembly of kojic acid into Zn-Ti layered double hydroxide with antibacterial and whitening performances. Chinese Chemical Letters, 30(4), 919–923.

Wawrzyk-Bochenek, I., Rahnama, M., Stachura, M., Wilczyński, S., Wawrzyk, A., 2023. Evaluation of the reduction of skin hyperpigmentation changes under the influence of a preparation containing kojic acid using hyperspectral imaging: Preliminary study. Journal of Clinical Medicine, 12(7).

Xue, F., Li, X., Qin, L., Liu, X., Li, C., Adhikari, B., 2021. Anti-aging properties of phytoconstituents and phyto-nanoemulsions and their application in managing aging-related diseases. Advanced Drug Delivery Reviews, 176, 113886.

Yuliani, S.H., Hartini, M., Pudyastuti, B., Istyastono, E.P., 2016. Comparison of physical stability properties of pomegranate seed oil nanoemulsion dosage forms with long-chain triglyceride and medium-chain triglyceride as the oil phase. Traditional Medicine Journal, 21(2), 94–98.

Zheng, Y., Zheng, M., Ma, Z., Xin, B., Guo, R., Xu, X., 2015. Sugar Fatty Acid Esters, Polar Lipids: Biology, Chemistry, and Technology. AOCS Press.

DOI: https://doi.org/10.24071/jpsc.009968