Inhibitory Effect of Ethanol Extract of Cherry Mistletoe Leaves (Dendrophthoe pentandra (L.) Miq) on Xanthine Oxidase Enzyme

Hana Daini Sabathania; Subandrate Subandrate; Sadakata Sinulingga; Safyudin Safyudin; Liniyanti D Oswari; Fatmawati Fatmawati

doi:10.24071/jpsc.008252

Inhibitory Effect of Ethanol Extract of Cherry Mistletoe Leaves (Dendrophthoe pentandra (L.) Miq) on Xanthine Oxidase Enzyme

Hana Daini Sabathania⁽¹⁾, Subandrate Subandrate^(2*), Sadakata Sinulingga⁽³⁾, Safyudin Safyudin⁽⁴⁾, Liniyanti D Oswari⁽⁵⁾, Fatmawati Fatmawati⁽⁶⁾,

(1) Medical Education Study Program, Faculty of Medicine, Universitas Sriwijaya
(2) Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya
(3) Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya
(4) Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya
(5) Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya
(6) Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya
(*) Corresponding Author

Abstract

One plant that can be used as an alternative treatment for hyperuricemia is the cherry plant. The usefulness of these plants is due to the secondary metabolite compounds that can inhibit the enzyme xanthine oxidase. Cherry mistletoe is thought to have the same flavonoids, saponins, alkaloids, terpenoids, and tannins as the cherry plant, which can act as inhibitors. The aim of the study was to determine the inhibitory effect of an ethanol extract of cherry mistletoe leaves on the xanthine oxidase enzyme. This study was conducted as an in vitro experimental study. Cherry mistletoe leaves were extracted with 96% ethanol. The extract was done with phytochemical screening, followed by an inhibition test on the xanthine oxidase enzyme using a spectrophotometer at a wavelength of 400 nm. Allopurinol was a positive control. The ethanol extract of cherry mistletoe leaves contains flavonoids, saponins, alkaloids, terpenoids, and tannins. The IC₅₀ value of the ethanol extract of cherry mistletoe leaves was 23.44 mg/L. The ethanol extract of cherry mistletoe leaves has an inhibitory effect on the xanthine oxidase enzyme with moderate activity. Accordingly, the cherry mistletoe leaf extract can be considered as an alternative inhibitor of the xanthine oxidase enzyme in the control of hyperuricemia.

Keywords

Cherry mistletoe leaves; Ethanol extract; Phytochemicals; Xanthine oxidase

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References

Alghamdi, Y.S., Soliman, M.M., Nassan, M.A., 2020. Impact of Lesinurad and allopurinol on experimental Hyperuricemia in mice: biochemical, molecular and Immunohistochemical study. BMC Pharmacology & Toxicology, 21(1), 10.

Anisa, N., Najib, S.Z., Yannas, A.F., Bangkalan, H., 2022. Skrining fitokimia dan penetapan kadar total fenol flavonoid dan tanin pada ekstrak etanol daun Kersen (Muntingia calabura L.). Indonesian Journal Pharmaceutical and Herbal Medicine, 1(2), 96–104.

Besharati, M., Maggiolino, A., Palangi, V., Kaya, A., Jabbar, M., Eseceli, H., De Palo, P., Lorenzo, J.M., 2022. Tannin in ruminant nutrition: Review. Molecules, 27(23).

Bhambhani, S., Kondhare, K.R., Giri, A.P., 2021. Diversity in chemical structures and biological properties of plant alkaloids. Molecules (Basel, Switzerland), 26(11).

Cao, H., Pauff, J.M., Hille, R., 2014. X-ray Crystal structure of a xanthine oxidase complex with the flavonoid inhibitor quercetin. Journal of Natural Products, 77(7), 1693–1699.

Danve, A., Sehra, S.T., Neogi, T., 2021. Role of diet in hyperuricemia and gout. Best Practice & Research. Clinical Rheumatology, 35(4), 101723.

Dari, D.W., Andika, A., Mirajunnisa, M., 2022. Uji potensi senyawa metabolit sekunder tanaman Putri Malu (Mimosa pudica L.) sebagai inhibitor xanthine oxidase secara in silico. Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 3(2), 171–183.

Diba, M.F., Salni, S., Subandrate, S., 2019. Cytotoxic test of extract and fraction of Dendrophtoe pentandra (L) Miq on T47D cell. Jurnal Kimia Sains dan Aplikasi, 22(3), 73.

Efendi, M.R.S., Trisnawati, A., 2022. Identification of secondary metabolites and xanthine oxidase inhibition of Kersen leaf extract (Muntingia calabura L). Journal of Chemistry, Education, and Science, 6(2), 146–152.

Fraga-Corral, M., García-Oliveira, P., Pereira, A.G., Lourenço-Lopes, C., Jimenez-Lopez, C., Prieto, M.A., Simal-Gandara, J., 2020. Technological application of tannin-based extracts. Molecules, 25(3).

Haryanta, D., 2023. Mengenal Tumbuhan Benalu pada Tanaman Hutan Kota di Surabaya. UWKS Press, Surabaya.

Hidayah, W.W., Kusrini, D., Fachriyah, E., 2016. Isolasi, Identifikasi Senyawa Steroid dari Daun Getih-Getihan (Rivina humilis L.) dan Uji Aktivitas sebagai Antibakteri. Jurnal Kimia Sains dan Aplikasi, 19(1),32-37.

, G.S., Suhud, F., 2021. Validation of in-vitro bioassay methods: Application in herbal drug research. Profiles of Drug Substances, Excipients, and Related Methodology, 46, 273–307.

Joosten, L.A.B., Crişan, T.O., Bjornstad, P., Johnson, R.J., 2020. Asymptomatic hyperuricaemia: A silent activator of the innate immune system. Nature Reviews Rheumatology, 16(2), 75–86.

Kong, D., Wang, L., Niu, Y., Cheng, L., Sang, B., Wang, D., Tian, J., Zhao, W., Liu, X., Chen, Y., Wang, F., Zhou, H., Jia, R., 2023. Dendrophthoe falcata (L.f.) Ettingsh. and Dendrophthoe pentandra (L.) Miq.: A review of traditional medical uses, phytochemistry, pharmacology, toxicity, and applications. Frontiers in Pharmacology, 14, 1096379.

Kregiel, D., Berlowska, J., Witonska, I., Antolak, H., Proestos, C., L, M.B., Babic, J., Zhang, B., 2017. Saponin-Based, Biological-Active Surfactants from Plants. IntechOpen.

Lin, S., Zhang, G., Liao, Y., Pan, J., 2015. Inhibition of chrysin on xanthine oxidase activity and its inhibition mechanism. International Journal of Biological Macromolecules, 81, 274–282.

Masriani, Budi, F.S., 2017. Penapisan Fitokimia Ekstrak Metanol Tumbuhan Obat Asal Kalimantan Barat, in: Seminar Nasional Ilmu Pengetahuan Alam Dan Teknologi. Universitas Tanjungpura, Potianak, 191–199.

Mohammed, H.A., Al-Omar, M.S., Mohammed, S.A.A., Alhowail, A.H., Eldeeb, H.M., Sajid, M.S.M., Abd-Elmoniem, E.M., Alghulayqeh, O.A., Kandil, Y.I., Khan, R.A., 2021. Phytochemical analysis, pharmacological and safety evaluations of halophytic plant, Salsola cyclophylla. Molecules (Basel, Switzerland), 26(8).

Mohos, V., Fliszár-Nyúl, E., Poór, M., 2020. Inhibition of xanthine oxidase-catalyzed xanthine and 6-mercaptopurine oxidation by flavonoid aglycones and some of their conjugates. International Journal of Molecular Sciences, 21(9).

Murdoch, R., Barry, M.J., Choi, H.K., Hernandez, D., Johnsen, B., Labrador, M., Reid, S., Singh, J.A., Terkeltaub, R., Vázquez Mellado, J., Dalbeth, N., 2021. Gout, Hyperuricaemia and Crystal-Associated Disease Network (G-CAN) common language definition of gout. RMD Open, 7(2).

Pan, Y., Lu, Z., Li, C., Qi, R., Chang, H., Han, L., Han, W., 2021. Molecular dockings and molecular dynamics simulations reveal the potency of different inhibitors against xanthine oxidase. ACS Omega, 6(17), 11639–11649.

Putri, N.E., Rissyelly, R., 2016. Uji penghambatan xantin oksidase secara in vitro ekstrak kulit rambutan. Pharmaceutical Sciences and Research, 3(1), 12–20.

Raharjo, D., 2022. Efektivitas penghambatan enzim xantin oksidase ekstrak etanol dan fraksi etanol, fraksi etil asetat serta fraksi N-heksane kulit Batang Mangrove Merah (Rhizopora Mucronata). Jurnal Ilmiah Kesehatan, 15(1), 63–70.

Safrida, S., Sabri, M., 2019. Effect of Muntingia calabura l. stem bark extracts on uric acid concentration and renal histopathology in diabetic rats. Medicina (Lithuania), 55(10).

Serrano, J.L., Figueiredo, J., Almeida, P., Silvestre, S., 2020. From xanthine oxidase inhibition to in vivo hypouricemic effect: An integrated overview of in vitro and in vivo studies with focus on natural molecules and analogues. Evidence-Based Complementary and Alternative Medicine, 2020, 9531725.

Sinulingga, S., Ng, M.S., Subandrate, S., Oswari, L.D., 2023. Nilai IC50 ekstrak dan fraksi daun Benalu Kersen terhadap enzim xantin oksidase. JSFK (Jurnal Sains Farmasi & Klinis), 10(1), 114–119.

Sinulingga, S., Subandrate, S., Safyudin, S., 2020. Uji fitokimia dan potensi antidiabetes fraksi etanol air Benalu Kersen (Dendrophtoe petandra (L) Miq). Jurnal Kedokteran dan Kesehatan, 16(1), 76.

Subandrate, Diba, M.F., Salni, Triwani, Nita, S., 2019. Cytotoxicity, antiproliferative and apoptotic effect of n-hexane fraction of lime parasite (Dendrophtoe pentandra). Molekul, 14(1).

Suwandi, D.W., Perdana, F., 2017. Aktivitas penghambatan xantin oksidase ekstrak etanol daun alpukat (Persea americana Mill) secara in vitro. Jurnal Ilmiah Farmako Bahari, 8(2), 40.

Thenmozhi, M., Arunthathi, K., Abhinav, J., Sharika, K.V., Dhasarathan, P., 2023. Extraction of phytochemical constituents from the leaves of Vitex trifolia in the inhibition of lipoxygenase, cyclooxygenase, tyrosinase and xanthine oxidase. Indian Journal of Agricultural Research, 58(2), 219-223.

Tien, Y.-Y., Shih, M.-C., Tien, C.-P., Huang, H.-K., Tu, Y.-K., 2022. To treat or not to treat? Effect of urate-lowering therapy on renal function, blood pressure and safety in patients with asymptomatic hyperuricemia: A systematic review and network meta-Analysis. Journal of the American Board of Family Medicine: JABFM, 35(1), 140–151.

Walid, M., Endriyatno, N.C., Amalia, R., 2023. Uji aktivitas antihiperurisemia ekstrak buah Kersen Hijau (Muntingia calabura L.) pada tikus jantan putih galur Wistar. Forte Journal, 3(2), 134–140.

Widjaya, S., Bodhi, W., Yudistira, A., 2019. Skrining fitokimia, uji aktivitas antioksidan, dan toksisitas dari ekstrak daun Kersen (Muntingia calabura L.) dengan metode E 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT). Pharmacon, 8(2), 315.

Xu, F., Zhao, X., Yang, L., Wang, X., Zhao, J., 2014. A new cycloartane-type triterpenoid saponin xanthine oxidase inhibitor from Homonoia riparia Lour. Molecules (Basel, Switzerland), 19(9), 13422–13431.

Xue, H., Xu, M., Gong, D., Zhang, G., 2023. Mechanism of flavonoids inhibiting xanthine oxidase and alleviating hyperuricemia from structure–activity relationship and animal experiments: A review. Food Frontiers, 4(4), 1643–1665.

Yang, Y., Laval, S., Yu, B., 2021. Chemical synthesis of saponins. Academic Press, 79, 63–150.

Yanti, P., Subarnas, A., Renggana, H., 2021. Review: Aktivitas antihiperurisemia beberapa tanaman dari Arboretum Garut. Jurnal Pharmascience, 8(2), 17–28.

Yulian, M., 2014. Potensi biodiversitas Indonesia sebagai inhibitor xantin oksidase dan antigout. Lantanida Journal, 2(1), 80.

DOI: https://doi.org/10.24071/jpsc.008252