Effectiveness of Nano Spray From Extract of Kelubut Leaf (Passiflora foetida L.) as Antibiofilm on catheters of Patients with Urinary Tract Infection

Hasyrul Hamzah; Nur Atika Astriani; Norma Feti Farida; Lila Zulfa Kamilah; Aryantinur Azizah; Fathur Rahman

doi:10.24071/jpsc.008198

Effectiveness of Nano Spray From Extract of Kelubut Leaf (Passiflora foetida L.) as Antibiofilm on catheters of Patients with Urinary Tract Infection

Hasyrul Hamzah^(1*), Nur Atika Astriani⁽²⁾, Norma Feti Farida⁽³⁾, Lila Zulfa Kamilah⁽⁴⁾, Aryantinur Azizah⁽⁵⁾, Fathur Rahman⁽⁶⁾,

(1) Universitas Muhammadiyah Kalimantan Timur
(2) Universitas Muhammadiyah Kalimantan Timur
(3) Universitas Muhammadiyah Kalimantan Timur
(4) Universitas Muhammadiyah Kalimantan Timur
(5) Universitas Muhammadiyah Kalimantan Timur
(6) Universitas Muhammadiyah Kalimantan Timur
(*) Corresponding Author

Abstract

Catheter-associated urinary tract infections (CAUTI) have increased significantly every year. Approximately 70-80% of these infections involve biofilms. This study aims to determine the effectiveness of nano spray formula of kelubut leaf extract in inhibiting and eradicating biofilm formation on catheters. Kelubut (Passiflora foetida L.) leaf extract was obtained through maceration process to obtain useful compounds. Antibiofilm activity was assessed using microbroth dilution, with scanning electron microscopy for visualization. The nano spray formulation activity of kelubut (Passiflora foetida L.) leaf extract was able to inhibit biofilm growth by (77.74% ± 0.01), while ciprofloxacin was slightly more effective with a value of (80.22% ± 0.01). SEM analysis confirmed the ability of the kelubut leaf extract nanospray formulation to inhibit and damage Escherichia coli biofilms. The nano spray formulation of kelubut leaf extract (Passiflora foetida L.) coded NS1 is able to inhibit biofilm formation activity on catheters and has the potential to be developed as an antibiofilm candidate against bacteria that cause urinary tract infections.

Keywords

Antibiofilm; Catheter; Escherichia coli; Passiflora foetida L.

Full Text:

PDF

References

Afrilina, I., Erly, E., & Almurdi, A. (2017). Identifikasi Mikroorganisme Penyebab Infeksi Saluran Kemih pada Pasien Pengguna Kateter Urine di ICU RSUP Dr. M. Djamil Padang Periode 01 Agustus-30 November 2014. Jurnal Kesehatan Andalas, 6(1), 196

Amyliana, N. A., & Agustini, R. (2021). Formulasi Dan Karakterisasi Nanoenkapsulasi Yeast Beras Hitam Dengan Metode Sonikasi Menggunakan Poloxamer. Unesa Journal of Chemistry, 10(2), 184–191

Baharlouei, P., & Rahman, A. (2022). Chitin and Chitosan : Prospective Biomedical Applications in. Marine Drugs, 20(7), 460.

Changestu, D. A., Asseggaf, S. N. Y. R. S., Hafizuddin, M., Alfazon, R., Yuannefa, N., & Kalahan, A. M. (2023). POTENSI AKTIVITAS ANTIBAKTERI DAUN RAMBUSA ( Passiflora foetida L . ) SEBAGAI PENGOBATAN TRADISIONAL ANTIBACTERIAL ACTIVITY POTENTIAL OF RAMBUSA LEAF ( Passiflora foetida L . ) AS TRADITIONAL MEDICINE. Indonesia Natural Research Pharmaceutical Journal, 8(2), 90–101.

Devi, S., & Mulyani, T. (2017). Uji Aktivitas Antibakteri Ekstrak Daun Pacar Kuku (Lawsonia inermis Linn) pada Bakteri Pseudomonas Aeruginosa. 1(1), 30–35.

Edwards JR, A.-B. K. D. M. (2015). National Healthcare Safety Network (NHSN) Report, Data Summary for 2013, Device-associated Module. Am J Infect Control, 43 (3), 206–221

Fahmy, R. W. S. M. H. M. A. A. L. I. (2020). Proteus mirabilis biofilm: Development and Therapeutic Strategies. Front Cell Infect Microbiol, 10, 414

Hamzah, H., Hertiani, T., Pratiwi, S. U. T., Nuryastuti, T., & Gani, A. P. (2020). Antibiofilm studies of zerumbone against polymicrobial biofilms of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and candida albicans. International Journal of Pharmaceutical Research, 12(1), 1307–1314

Hamzah, H., Pratiwi, S. U. T., & Hertiani, T. (2022). Efficacy of C-10 massoialactone against-multispecies microbial biofilm. Biointerface Research in Applied Chemistry, 12(3), 3472–3487

Hamzah, H., Pratiwi, S. U. T., Jabbar, A., Hafifah, A. S., Al-Fajri, B. A., & Nurhalisah, N. (2023). Bioactivity Tracing of the Ethanol Extract of Bajakah Tampala (Spatholobus littoralis Hassk.) Typical Plant of Kalimantan Island as Antibiofilm of Staphylococcus aureus. Open Access Macedonian Journal of Medical Sciences, 11(A), 8–14

Hamzah, H., Siregar, K. A. A. K., Suffiana, Y., Yudhawan, I., & Nurwijayanto, A. (2022). Antibacterial and antibiofilm activity of Begonia multangula Blume. leaf extract against Candida albicans. Food Research, 6(1), 260–268

Hamzah, H., Yudhawan, I., Rasdianah, N., Setyowati, E., Nandini, E., & Pratiwi, S. U. T. (2022). Clove oil has the activity to inhibit middle, maturation and degradation phase of candida tropicalis biofilm formation. Biointerface Research in Applied Chemistry, 12(2), 1507–1519

Harjai, K. S. S. K. B. S. C. K. (2020). Exploring the therapeutic efficacy of zingerone nanoparticles in treating biofilm- associated pyelonephritis caused by Pseudomonas aeruginosa in the murine model. Inflammation, 43, 2344–2356

Hertiani, H. H. S. U. T. P. T. (2018). Efficacy of thymol and eugenol against polymicrobial biofilm. Indonesian Journal of Pharmacy, 29 (4), 214

Juliantoni, Y., Hajrin, W., & Subaidah, W. A. (2020). Nanoparticle Formula Optimization of Juwet Seeds Extract (Syzygium cumini) using Simplex Lattice Design Method. Jurnal Biologi Tropis, 20(3), 416–422

Maulana, I. A., Triatmoko, B., & Nugraha, A. S. (2020). Skrining Fitokimia dan Uji Aktivitas Antibakteri Ekstrak dan Fraksi Tanaman Senggugu (Rotheca serrata (L.) Steane & Mabb.) terhadap Pseudomonas aeruginosa. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 5(1), 01

Mohammed, M. A., Syeda, J. T. M., Wasan, K. M., & Wasan, E. K. (2017). An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics, 9(4)

Nandini, H. H. S. U. T. P. (2022). Efficacy of Bajakah Tampala ethanol extract, a typical plant of Kalimantan Island (Borneo), against candida albicans biofilm. European Chemical Bulletin, 11(5)

Nicolle. (2014). Catheter Associated Urinary Tract Infections. Antimicrobial Resistance and Infection Control, 3, 23

Ode, L., Nur, A., Oleo, U. H., Radiman, C. L., Wahyuningrum, D., & Suendo, V. (2010). Deasetilasi Kitin secara Bertahap dan Pengaruhnya terhadap Derajat Deasetilasi serta Massa molekul Kitosan. Jurnal Kimia Indonesia, 5(January), 17–21.

Rachmawati, A. L., & Surini, S. (2018). Formulasi dan Karakterisasi Nanopartikel Sambungsilang Gom Xantan dan Gom Akasia Untuk Penghantaran Insulin Oral. Pharmaceutical Sciences and Research, 5(3), 159–168

Román-Doval, R., Torres-Arellanes, S. P., Tenorio-Barajas, A. Y., Gómez-Sánchez, A., & Valencia-Lazcano, A. A. (2023). Chitosan: Properties and Its Application in Agriculture in Context of Molecular Weight. Polymers, 15(13), 1–26

Sari, G. N. F., & Puspitasari, I. (2021). AKTIVITAS ANTIBAKTERI DAN BIOAUTOGRAFI EKSTRAK DAUN RAMBUSA (Passiflora foetida L) TERHADAP Pseudomonas aeruginosa-Klebsiella pneumoniae. Media Farmasi: Jurnal Ilmu Farmasi, 18(2), 102

Smith, M. K. K. H. (2017). Microbiology : A systems Approach(5 th ed). In McGraw-Hill Education.

Wang, L., Yang, J., Yang, X., Hou, Q., Liu, S., Zheng, W., Long, Y., & Jiang, X. (2020). Mercaptophenylboronic Acid-Activated Gold Nanoparticles as Nanoantibiotics against Multidrug-Resistant Bacteria. ACS Applied Materials and Interfaces, 12(46), 51148–51159

Werneburg, G. T. (2022). Catheter-Associated Urinary Tract Infections: Current Challenges and Future Prospects. Research and Reports in Urology, 14(March), 109–133

Wijianto, B., & Hamzah, H. (2022). Efficacy of Onchidiid Slug (Onchidiium Typhae) Ethanolic Extract Against Bacterial and Fungal Grown in Biofilm Cultures. European Chemical Bulletin, 11(10), 112–116

Wirasti, Rahmatullah, S., Slamet, Permadi, Y. W., & Agmarina, S. N. (2021). PENGUJIAN KARAKTER NANOPARTIKEL METODE GELASI IONIK EKSTRAK DAN TABLET DAUN AFRIKA ( Vernonia TESTING OF NANOPARTICLE IONIC GELATION METHOD OF EXTRACT AND TABLET OF AFRIKA LEAT ( Vernonia Amygdalina Del .). Jurnal Wiyata, 147–151.

DOI: https://doi.org/10.24071/jpsc.008198