Nanoemulgel Formulation of Eleutherine palmifolia Onion Bulb Extract and Its Effectiveness in Wound Healing

Rini Dwiastuti; Muhammad Faisal; Lutfi Chabib; Melynia Sintha Dewi; Michael Raharja Gani

doi:10.24071/jpsc.007454

Nanoemulgel Formulation of Eleutherine palmifolia Onion Bulb Extract and Its Effectiveness in Wound Healing

Rini Dwiastuti^(1*), Muhammad Faisal⁽²⁾, Lutfi Chabib⁽³⁾, Melynia Sintha Dewi⁽⁴⁾, Michael Raharja Gani⁽⁵⁾,

(1) Faculty of Pharmacy, Universitas Sanata Dharma
(2)
(3)
(4)
(5)
(*) Corresponding Author

Abstract

Eleutherine palmifolia onion, an indigenous medicinal plant from Borneo, is empirically applied to treat wound healing. The objective of this study is to formulate its extract and assess its in vivo wound healing activity. Eleutherine palmifolia onion was extracted with a 96% ethanol solvent. The extract of the Dayak onion bulb was then formulated into a nanoemulsion by applying the DOE method of 2-level factorial design. Four nanoemulsion formulations were further analyzed to obtain the optimal formulation. The particle size analysis and % transmittance was performed using a particle size analyzer (PSA) and an ultraviolet-visible spectrophotometer. Furthermore, the data were analyzed using the Minitab.19 application with the DOE factorial design method. The three nanoemulgel formulations containing 2.5, 5.0, and 7.5% extract further investigated wound healing efficacy in rats. The duration of wound closure was analyzed using paired t-tests. The best nanoemulsion formulation was made with 22.5 mL of Tween 80 and 2 mL of isopropyl myristate oil. This produced particles that were 12.13 nm in size and a 99.12% transmittance rate. Wound length measurements were taken daily for 15 days. The statistical test results of p<0.05 indicate that the nanoemulgel of the extract has activity in wound healing. The optimal nanoemulsion formula is FA, and the effective dose of Dayak onion extract nanoemulgel is 5% and 7.5% concentration, with a wound healing time of 13 days.

Keywords

Dayak onion bulb; factorial design, particle size analyzer; rats

References

Abdulai, I.L., Kwofie, S.K., Gbewonyo, W.S., Boison, D., Puplampu, J.B., Adinortey, M.B., 2021. Multitargeted Effects of Vitexin and Isovitexin on Diabetes Mellitus and Its Complications. The Scientific World Journal, 2021, 1–20.

Ananta, G.P., 2020. Potensi Batang Pisang (Musa Pardisiaca L.) Dalam Penyembuhan Luka Bakar. Jurnal Ilmiah Kesehatan Sandi Husada, 11(1): 334–340.

Ayuningtyas, N. D., Solichah, A. I., Fadhilah, R. N., Subekti, T., 2022. Formulation and Antimicrobial Activity of Jeringau Leaves Extract (Acorus calamus Linn.) Nanoemulsion Mouthwash. Jurnal Sains Dan Kesehatan, 4(SE-1), 93–100.

Calsum, U., Khumaidi, A., Khaerati, K., 2018. Aktivitas Ekstrak Etanol Kulit Batang Kayu Jawa (Lannea coromandelica) terhadap Penyembuhan Luka Sayat pada Tikus Putih (Rattus Norvegicus L.). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy), 4(2): 113–118.

Christania, F.S., Dwiastuti, R., Yuliani, S.H., 2020. Lipid And Silver Nanoparticles Gels Formulation Of Tempeh Extract. Journal of Pharmaceutical Sciences and Community, 16(2): 56–62.

Dwiastuti, R., Gumilar, K.N.B., Yuliani, S.H., 2023. Optimization and Physical Characeterization of Quercetin Nanoemulgel Formula as an Antibacterial Agent. International Journal of Applied Pharmaceutics, 15(1): 104–110.

Febrinda, A.E., Astawan, M., Wresdiyati, T., Dewi Yuliana, N., 2013. Kapasitas Antioksida dan Inhibitor Alfa Glukosidase Ekstrak Umbi Bawang Dayak. Jurnal Teknologi dan Industri Pangan, 24(2): 161–167.

Fitri, D., Kiromah, N.Z.W., Widiastuti, T.C., 2020. Formulasi Dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Salam (Syzygium polyanthum) Pada Berbagai Variasi Komposisi Kitosan Dengan Metode Gelasi Ionik. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 5(1): 61-69.

Khairiah, A., Kusuma E.W., 2021. Uji Aktivitas Anti-Inflamasi Krim Ekstrak Etanol Bawang Dayak (Eleutherine Palmifolia (L) Merr) Pada Luka Sayatan Tikus Putih Jantan. Jurnal Ilmiah Manuntung, 7(2):158–64.

Kumalasari, E., Agustina, D., Ariani, N., 2020. Uji Aktivitas Antibakteri Ekstrak Daun Bawang Dayak (Eleutherine Palmifolia Merr.) Terhadap Escherichia coli. Jurnal Insan Farmasi Indonesia, 3(1):75–84.

Kumowal, S., Fatimawali, F., Jayanto, I., 2019. Uji Aktivitas Antibakteri Nanopartikel Ekstrak Lengkuas Putih (Alpinia galanga (L.) Willd) terhadap Bakteri Klebsiella pneumoniae. Pharmacon: Jurnal Farmasi Indonesia, 8(4): 781–790.

Lallo, S., Hardianti, B., Umar, H., Trisurani, W., Wahyuni, A., Latifah, M., 2020. Aktivitas Anti Inflamasi dan Penyembuhan Luka dari Ekstrak Kulit Batang Murbei (Morus alba L.). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy), 6(1): 26–36.

Muti’ah, R., Listiyana, A., Nafisa, B.B., Suryadinata, A., 2020. Kajian Efek Ekstrak Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr) sebagai Antikanker. Journal of Islamic Pharmacy, 5(2): 14–25.

Nastiti, C.M.R.R., Dwiastuti, R., Riswanto, F.D.O., 2023. Novel Quercetin Nanoemulgel Optimization: Gelling Agents Evaluation and The Application of Response Surface Methodology. International Journal of Applied Pharmaceutics, 15(1): 72–78.

Novaryatiin, S., Pratiwi, A.M., Ardhany, S.D., 2018. Uji Daya Hambat Ekstrak Etanol Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) Terhadap Bakteri Staphylococcus epidermidis. Anterior Jurnal, 18(1): 92–97.

Nurleni, N., Erviana, N., Firdiawan, A., Sari, E.R., 2023. Formulasi Sediaan Krim Asam Kojat Dengan Variasi Isopropil Miristat Sebagai Enhancer dan Evaluasi Stabilitas Fisika Waktu Sebenarnya. Jurnal Ilmiah Bakti Farmasi, 7(2): 9–14.

Nursal, F., Sumirtapura, Y., Suciati, T., Kartasasmita, R., 2019. Optimasi Nanoemulsi Natrium Askorbil Fosfat melalui Pendekatan Design of Experiment (Metode Box Behnken). Jurnal Sains Farmasi & Klinis, 6(3): 228-236.

Pariyana, P., Saleh, M., Tjekyan, S., Hermansyah, H., 2018. Efektivitas Pemberian Ekstrak Daun Binahong (Anredera Cordifolia) Terhadap Ketebalan Jaringan Granulasi dan Jarak Tepi Luka pada Penyembuhan Luka Sayat Tikus Putih (Rattus Norvegicus). Jurnal Kedokteran dan Kesehatan: Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya, 3(3): 155-165.

Peng, Y., Gan, R., Li, H., Yang, M., McClements, D.J., Gao, R., Sun, Q., 2021. Absorption, metabolism, and bioactivity of vitexin: recent advances in understanding the efficacy of an important nutraceutical. Critical Reviews in Food Science and Nutrition, 61(6): 1049–1064.

Priani, S.E., Wulansari, D.Y., Darusman, F., 2021. Formulasi Sediaan Nanoemulsi Antiselulit Mengandung Kafein dan Minyak Biji Anggur (Vitis vinifera L.). Jurnal Ilmu Kefarmasian Indonesia, 19(2):231–6.

Putri, S.S., Suryati, C., Nandini, N., 2020. Formulasi dan Uji Aktivitas Antibakteri Nanoemulsi Mouthwash Ekstrak Etanol Daun Jeringau (Acorus calamus Linn.). Jurnal Sains dan Kesehatan, 3(1):242–7.

Qushawy, M., Nasr, A., 2019. Solid Lipid Nanoparticles (SLNs) As Nano Drug Delivery Carriers: Preparation, Characterization and Application. International Journal of Applied Pharmaceutics, 12(1): 1–9.

Sari, D.P., Nahzi, M.Y.I., Budiarti, L.Y., 2017. Efektivitas Daya Hambat Ekstrak Umbi Bawang Dayak Terstandarisasi Fenol Terhadap Pertumbuhan Enterococcus Faecalis. Dentin: Jurnal Kedokteran Gigi, 21(1): phar56–61.

Singh, A., Kumar, R., Kannaujia, S.K., Singh, N.P., 2022. Haematobiochemical and Histopathological Studies on the Effects of Raj Nirwan Bati (a Novel Allovedic Drug) in Wistar Rats. International Journal of Pharmacy and Pharmaceutical Sciences, 14(2): 21–30.

Setiawan, A., Kiromah, N.Z.W., Widiastuti, T.C., 2020. Formulasi dan Evaluasi Sediaan Tablet Nanopartikel Ekstrak Daun Salam (Syzygium Polyanthum) dengan Variasi Konsentrasi Na Alginat dan Avicel PH 102. Pharmacon: Jurnal Farmasi Indonesia, 17(1): 24–30.

Seja, Y., Ardana, M., Aryati, F., 2018. Pengaruh Suhu dan Lama Penyimpanan Ekstrak Bawang Dayak (Eleutherine americana L (Merr)) terhadap Aktivitas Antibakteri. Proceeding of Mulawarman Pharmaceuticals Conferences, 8(1): 150–155.

Soegiharto, C., Pratiwi, R., Wardhana, E.S., 2022. Efektivitas Nanoemulsi Gel Daun Pegagan (Centella Asiatica L.) Konsentrasi 25 %, 50 % Dan 75 % Terhadap Ketebalan Biofilm Bakteri Staphylococcus Aureus (In Vitro). Prosiding Konstelasi Ilmiah Mahasiswa Unissula, 7:94–101.

Subair, T.K., Mohanan, J., 2022. Development Of Nano Based Film Forming Gel for Prolonged Dermal Delivery Of Luliconazole. International Journal of Pharmacy and Pharmaceutical Sciences, 14(2): 31–41.

Suciati, T., Prihantini, M., Fidrianny, I., 2019. Optimasi Nanoemulsi A/M/A Ekstrak Etanol Daun Binahong dan Konjugat AG-Kitosan Menggunakan Desain Box-Behnken. Jurnal Ilmu Kefarmasian Indonesia, 17(2):150–9.

Wigati, D., Rahardian, R.R., 2018. Penetapan Standarisasi Non Spesifik Ekstrak Etanol Hasil Perkolasi Umbi Bawang Dayak (Eleutherine Palmifolia (L.) Merr). JIFFK: Jurnal Ilmu Farmasi dan Farmasi Klinik, 15(2), 36–40.

Wijayanti, S., Hasyati, N., 2018. Potensi Ekstrak Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr.) Dalam Mencegah Ulcerative Colitis Pada Mencit Yang Diinduksi DSS (Dextran Sulphate Sodium). Jurnal Ilmu Pangan dan Hasil Pertanian, 2(1): 40-52.

Wulansari, S.A., Sumiyani, R., Aryani, N.L.D. 2019. Pengaruh Konsentrasi Surfaktan Terhadap Karakteristik Fisik Nanoemulsi dan Nanoemulsi Gel Koenzym Q10. Jurnal Kimia Riset, 4(2): 143–151.

Zu, Y., Zhang, Q., Zhao, X., Wang, D., Li, W., Sui, X., Zhang, Y., Jiang, S., Wang, Q., Gu, C., 2012. Preparation and characterization of vitexin powder micronized by a supercritical antisolvent (SAS) process. Powder Technology, 228: 47–55.

Ananta, G.P., 2020. Potensi Batang Pisang (Musa Pardisiaca L.) Dalam Penyembuhan Luka Bakar. Jurnal Ilmiah Kesehatan Sandi Husada, 11(1): 334–340.

Christania, F.S., Dwiastuti, R., Yuliani, S.H., 2020. Lipid And Silver Nanoparticles Gels Formulation Of Tempeh Extract. Journal of Pharmaceutical Sciences and Community, 16(2): 56–62.

Wulansari, S.A., Sumiyani, R., Aryani, N.L.D. 2019. Pengaruh Konsentrasi Surfaktan Terhadap Karakteristik Fisik Nanoemulsi dan Nanoemulsi Gel Koenzym Q10. Jurnal Kimia Riset, 4(2): 143–151.

DOI: https://doi.org/10.24071/jpsc.007454