Antihyperlipidemic Effectivity of Sweet Orange Peel Extract (Citrus sinensis) on Triglyceride Levels in Male Mice (Mus musculus) Induced by High Cholesterol

Ellya Catharine Romanna Aprilya Lumbantoruan(1), Mistika Zakiah(2), Andhi Fahrurroji(3*),

(1) Faculty of Medicine, Universitas Tanjungpura, Pontianak, 78124, Indonesia
(2) Department of Pharmacology, Faculty of Medicine, Universitas Tanjungpura, 78124, Indonesia
(3) Department of Pharmaceutical Technology, Faculty of Medicine, Universitas Tanjungpura, 78124, Indonesia
(*) Corresponding Author

Abstract


Hyperlipidemia is a significant risk factor for atherosclerosis in arteries and cardiovascular disease, especially coronary heart disease (CHD), the leading cause of global mortality. Treatment of hyperlipidemia can be done through chemical treatment. However, statins have potentially dangerous side effects such as myopathy and rhabdomyolysis, so a therapeutic solution with minimal toxicity is needed. One of the potential treatments is Citrus sinensis orange peel, which contains antihyperlipidemic bioactive compounds, namely hesperidin, and naringenin. The aim of this research is to determine the effectivity of sweet orange peel extract (Citrus sinensis) on high cholesterol-induced male mice (Mus musculus) triglyceride levels. Citrus sinensis orange peel was extracted by maceration method using 50% ethanol as solvent. Lipid profile measurements were performed three times using the GPO-PAP method. The concentration of orange peel extract in the treatment group (P1, P2, P3) was 10%, 5%, and 1%. The positive control received 40 mg atorvastatin treatment, the negative control received 1% CMC Na, and the control received abnormal treatment. Antihyperlipidemic therapy with Citrus sinensis sweet orange peel extract has a strong relationship/influence on triglyceride levels in mice. Sweet orange peel extract (Citrus sinensis) has antihyperlipidemic effectiveness in reducing blood triglyceride levels in mice (Mus musculus) induced by high cholesterol. The effective dose is 1% dose.

Keywords


Antihyperlipidemia; Citrus sinensis peel; Mice; Obesity; Triglycerides

Full Text:

PDF

References


Ahn, E. M., Myung, N.-Y., Jung, H.-A., Kim, S.-J. 2019. The ameliorative effect of Protaetia brevitarsis larvae in HFD-induced obese mice. Food Science and Biotechnology, 28(4), 1177-1186.


Ardiani, R. 2017. Anticholesterol effect of ethanol extract of African leaves (Vernonia amygdalina del.) in rats. MIPA Education Research Journal, 2(1), 153-158.


Ardiansyah, S. A., Hidayat, D. S., Simbolon, N. S. 2017. Test the antiobesity activity of the ethanol extract of Malacca leaves (Phyllanthus emblica L.) against male white rats Wistar strain. Journal of Indonesian Pharmaceutical Science and Technology, 7(1), 27.


Chen, Z. T., Chu, H. L., Chyau, C. C., Chu, C. C., Duh, P. 2013. Protective effects of sweet orange (Citrus sinensis) peel and their bioactive compounds on oxidative stress. Food Chemistry, 135(4), 2119–2127.


Dulcich, M.S. 2013. The effects of proton radiation and pomegranates on hippocampus and behavior. Loma Linda University.


Dwizella, N., Berawi, K. N., Wahyudo, R. 2018. Efficacy of rice bran in lowering blood fat levels in hyperlipidemia patients. Journal of Majority, 7(2), 209–213.


Etebu, E., Nwauzoma, A.B. 2014. A review on sweet orange (Citrus sinensis L Osbeck): Health, diseases and management. American Journal of Research Communication, 2(2), 33-70.


Gunawan, S.G., Nafriadi. 2013. Pharmacology and therapeutics (5th ed.). FK UI.


Iqbal Arief, M., Novriansyah, R., Tjeng Budianto, I., Harmaji, B. 2013. Potential of Karamunting flowers (Melastoma malabathricum L.) on total cholesterol and triglyceride levels in propylthiouracil-induced hyperlipidemic male rats. Achievement Journal, 1(2), 37-39.


Ji, X., Shi, S., Liu, B., Shan, M., Tang, D., Zhang, W., Zhang, H., Lu, C., Wang, Y. 2019. Bioactive compounds from herbal medicines to manage dyslipidemia. Journal of Biomedicine and Pharmacotherapy, 118, 1–12.


Kartikasari, D., Kharisma Justicia, A., Endang, P. 2019. Determination of total flavonoid content in ethanol extracts of red andong leaves and green andong leaves. Journal of Indonesian Pharmacists, 2(1), 108–117.


Leamy, L. J., Pomp, D., Lightfoot, J. T. 2019. Genetic variation for body weight change in mice in response to physical exercise. BMC Genetics, 10(1), 1-11.


Mallick, N., Khan, R. A. 2016. Antihyperlipidemic effects of Citrus sinensis, Citrus paradisi, and their combinations. Journal of Pharmacy and Bioallied Sciences, 8(2), 112–118.


Megantara, I. N. A. P., Megayanti, K., Wirayanti, R., Esa, I.B.D., Wijayanti, N.P.A.D., Yustiantara, P.S., Megayanti, K. 2017. Lotion formulation of raspberry fruit extract (Rubus rosifolius) with various concentrations of triethanolamine as an emulsifier and hedonic test on lotion. Udayana Pharmacy Journal, 6(1), 1-5.


M’hiri, N., Ioannou, I., Ghoul, M., Boudhrioua, N. M. 2014. Extraction methods of citrus peel phenolic compounds. Food Reviews International, 30(4), 265-290.


National Heart, Lung, and Blood Institute. 2020. Aim for a Healthy Weight. National Institutes of Health.


Putri, T. A., Ruyani, A., Nugraheni, E. 2017. Testing the effect of methanol extract of beluntas leaves (Pluchea indica l.) on blood glucose and triglyceride levels of mice (Mus musculus) induced by sucrose. Raflesia Medical Journal, 3(1).


Rekha, S. S., Pradeepkiran, J. A., Bhaskar, M. 2019. Bioflavonoid hesperidin possesses anti-hyperglycemic and hypolipidemic property in STZ induced diabetic myocardial infarction (DMI) in male Wister rats. Journal of Nutrition and Intermediary Metabolism, 15, 58–64.


Rial, S. A., Jutras-Carignan, A., Bergeron, K.-F., Mounier, C. 2020. A high-fat diet enriched in medium chain triglycerides triggers hepatic thermogenesis and improves metabolic health in lean and obese mice. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1865(3), 158582.


Setyaji, D. Y., Prabandari, Y. S., Gunawan, I.M.A. 2018. Physical activity with coronary heart disease in Indonesia. Indonesian Journal of Clinical Nutrition, 14(3), 115–121.


Widuri, C. K. 2018. Evaporation and characterization of concentrated mozzarella cheese whey from a rotary vacuum evaporator at a temperature range of 40°C-50°C [Thesis]. Brawijaya University.




DOI: https://doi.org/10.24071/jpsc.005944

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community)

 

 

 

 

 

 

 

  

Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community)

Published by Faculty of Pharmacy, Universitas Sanata Dharma Yogyakarta

Creative Commons Licence
This work is licensed under a Creative Commons Attribution 4.0 International License.