Filiana Santoso, Jennifer Sunardi, Florence Ignatia, Maria Dewi Puspitasari Tirtaningtyas Gunawan-Puteri


Functional beverages from lemongrass (Cymbopogon citratus), white ginger (Zingiber officinale Roscoe) and black tea (Camelia sinensis) were developed based on their α-glucosidase inhibitory (AGI) activities and sensory acceptance. The AGI was evaluated using in vitro enzymatic assay, while sensory acceptance was tested using affective sensory tests. The evaluation of their aqueous extracts showed that dried lemongrass and ginger possessed higher extraction yield (3.4 %, 2.7 %, respectively), though not necessarily accompanied with a better AGI activity (IC50 24.50 mg/mL, IC50 16.61 mg/mL) than the fresh lemongrass and ginger (2.1 %, 1.8 %, IC50 17.93 mg/ml, IC50 >47.00 mg/mL, respectively). Meanwhile, the evaluation of the combined extract showed additive and synergistic effects. The extract combination formula was selected based on the sensory acceptance, resulting in the beverages containing 4.29 mg/mL of lemongrass, 0.71 mg/mL of ginger, and 1.05 mg/mL of black tea with a total phenolic content of 636.45 mg/L Gallic Acid Equivalent (GAE). The selected formula showed the stability of AGI activity andthe pH value at 4 °C were in accordance with the growth of microbial count that was lower than those stored at 25 °C in a 50-day period. Changes in color and oBrix value were not significantly observed in the samples stored at 25 °C and 4 °C. Lime juice was selected as the additional flavoring agent, which could increase both the palatability and AGI activity of the beverages.


alpha glucosidase inhibitor; diabetes; functional food; ginger; lemongrass

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Abdulrazaq, N.B., Cho, M.M., Win, N.N., Zaman, R., Rahman, M.T., 2012. Beneficial Effects of Ginger (Zingiber officinale) on Carbohydrate Metabolism in Streptozotocin-induced Diabetic Rats. British Journal of Nutrition, 108(7), 1194–1201.

Ademuyiwa, A.J., Olamide, O.Y., Oluwatosin, O.O., 2015. The Effects of Cymbopogon citratus (Lemongrass) on the Blood Sugar Level, Lipid Profiles and Hormonal Profiles of Wistar Albino Rats. Merit Research Journal of Medicine and Medical Science, 3(6), 210–216.

Al-samydai, A., Al-mamoori, F., Shehadeh, M., Hudaib, M., 2018. Anti – Diabetic Activity of Cinnamon: A Review. International Research Journal of Pharmacy and Medical Sciences, 1(5), 43–45.

Amiri, A., Azemi, M.E., Khodayar, M.J., Namjoyan, F., 2015. In Vitro α- Amylase and α- Glucosidases Inhibitory Effects of Some Plant Extracts. International Journal of Pharmacognosy and Phytochemical Research, 7(2), 315-318.

Andallu, B., Radhika, B., Suryakantham, V., 2003. Effect of Aswagandha, Ginger and Mulberry on Hyperglycemia and Hyperlipidemia. Plant Foods for Human Nutrition, 58, 1–7.

Azzeh, F.S., 2013. Synergistic Effect of Green Tea, Cinnamon and Ginger Combination on Enhancing Postprandial Blood Glucose. Pakistan Journal of Biological Sciences, 16(2), 74–79.

Bharti, S.K., Kumar, A., Prakash, O., Krishnan, S., Gupta, A.K., 2013. Essential Oil of Cymbopogon citratus Against Diabetes: Validation by In vivo Experiments and Computational Studies. Journal of Bioanalysis and Biomedicine, 5(5), 194–203.

Christianty, F.M., Holidah, D., Yasmin, 2016. In vitro α-Glucosidase Inhibitory Activity of Various Tea (Camellia sinensis L.) Extracts. Proceeding ICMHS, 104–107.

Ellis, H., 2002. Tea: Discovering, Exploring, Enjoying, p 6-60. Ryland Peters & Small, New York.

Food and Drug Administration (FDA), 2005. Guidance for industry: estimating the maximum safe starting dose in initial clinical trials for therapeutics in adult healthy volunteers. Center for Drug Evaluation and Research (CDER), United States.

Garba, H.A., Mohammed, A., Ibrahim, M.A., Shuaibu, M.N., 2020. Effect of Lemongrass (Cymbopogon citratus Stapf) Tea in a Type 2 Diabetes Rat Model. Clynical Phytoscience, 6(19), 1–10.

Gardner, E.J., Ruxton, C.H.S., Leeds, A.R., 2007. Black tea - Helpful or Harmful? A Review of The Evidence. European Journal of Clinical Nutrition, 61(1), 3–18.

Gunawan-Puteri, M.D.P.T., Kartawiria, I.S., Widiputri, D.I., 2017. Aqueous Extraction Optimization of C. citratus for Development of Food Ingredients with Alpha Glucosidase Inhibitory. In Sukmana, I. and Ulvan, A. Integrated Sci- Tech: Interdisciplinary Research Approach, (2), 55-61.

Gunawan-Puteri, M.D.P.T., Rustandi, F., Hendra, P., 2018. Spray Dried Aqueous Extract of Lemongrass (Cymbopogon citratus) Exhibits In-vitro and In-vivo Anti-hyperglycaemic Activities. Journal of Pharmaceutical Science and Community, 15(2), 55–61.

International Diabetes Federation. IDF Diabetes Atlas, 9th edn. Brussels, Belgium.

Jumepaeng, T., Luthria, D., Chanthai, S., 2012. The Effect of Surfactant on Headspace Single Drop Microextraction for the Determination of Some Volatile Aroma Compounds in Citronella Grass and Lemongrass Leaves by Gas Chromatography. Analytical Method, 4, 421-428.

Lee, W.K., Wong, L.L., Loo, Y.Y., Kasapis, S., Huang, D., 2010. Evaluation of different teas against starch digestibility by mammalian glycosidases. Journal of Agricultural and Food Chemistry, 58(1), 148–154.

Malongane, F., McGaw, L., Mudau, F.N., 2017. The Synergistic Potential of Various Teas, Herbs and Therapeutic Drugs in Health Improvement. Journal of the Science of Food and Agriculture, 97(14), 4679–4689.

Marcus, J.B., 2019. Aging, Nutrition and taste: Nutrition, Food Science and Culinary Perspectives for Aging Tastefully. Academic Press, San Diego.

Mawarti, H., As’ad, Z.M., Rajin, M., 2018. Ameliorative Effect of Citrus aurantifolia and Cinnamomum burmannii Extracts on Diabetic Complications in A Hyperglycaemic Rat Model. Tropical Journal of Pharmaceutical Research, 17(5), 823–829.

Mun’im, A., Katrin, Azizahwati, Andriani, A., Mahmudah, K.F., Mashita, M., 2013. Screening of α- Glucosidase Inhibitory Activity of Some Indonesian Medicinal Plants. International Journal of Medicinal and Aromatic Plants, 3(2), 144–150.

Oboh, G., Akinyemi, A.J., Ademiluyi, A.O., Adefegha, S.A., 2010. Inhibitory Effects of Aqueous Extracts of Two Varieties of Ginger on Some Key Enzymes Linked to Type-2 Diabetes In Vitro. Journal of Food and Nutrition Research, 49(1), 14-20.

Poh, K.H., Muhammad, N., Abdullah, N., Talip, B.A., 2018. The Evaluation of Antioxidant Activity of Individual and Mixture of Lemongrass, Curry Leaves, Turmeric and Ginger Extracts. Journal of Science and Technology, 10(2), 66–70.

Priyarani, M., Padmakumari, P., Sankarikutty, B., Cherian, O.L., Nisha, V.M., Raghu, K.G., 2011. Inhibitory Potential of Ginger Extracts Against Enzymes Linked to Type 2 Diabetes, Inflammation and Induced Oxidative Stress Inhibitory. International Journal of Food Sciences and Nutrition, 62(2), 106–110.

Sahamastuti, A.A.T., Andre, Foustine, S., Sumarpo, A., Hartiadi, L.Y., 2019. Synergistic Antibacterial Activities of Ginger and Lemongrass Essential Oils as an Alternative Prevention to Food-Borne Disease. Indonesian Journal of Life Sciences, 01(02), 54–61.

Santoso, F., 2018. Application of Lemongrass (Cymbopogon citratus) as a Functional Food Ingredient with Alpha- Glucosidase Inhibitory Activity. Advance in Engineering Research, 172, 205–209.

Singh, A.B., Singh, N., Maurya, R., Kumar, A., 2009. Anti-hyperglycaemic, Lipid Lowering and Anti-oxidant Properties of [6]-gingerol in db/db Mice. International Journal of Medicine and Medical Sciences, 1(12), 536–544.

Singh, R.P., and Heldman, D.R., 2014. Dehydration, in: Introduction to Food Engineering. Fifth Edition. Academic Press, San Diego.

Togatorop, D.M., Nainggolan, R.J., Lubis, L.M., 2015. Pengaruh Perbandingan Sari Batang Sereh dengan Sari Jahe Dan Konsentrasi Serbuk Gula Aren Terhadap Mutu Serbuk Minuman Penyegar Sereh. Jurnal Rekayasa Pangan dan Pertanian, 3(2), 157–163.

Ukers W.H.,1935. All about tea. The Tea and Coffee Trade Journal Company, New York.

Wang, S., Meckling, K.A., Marcone, M.F., Kakuda, Y., Tsao, R., 2011. Synergistic, Additive, and Antagonistic Effects of Food Mixtures on Total Antioxidant Capacities. Journal of Agricultural and Food Chemistry, 59(3), 960–968.

Widiputri, D.I., Gunawan-Puteri, M.D.P.T, Kartawiria, I.S., 2018. Benchmarking Study of Cymbopogon citratus and C. nardus for Its Development of Functional Food Ingredient for Anti-diabetic Treatment. Proceedings of the International Conference on Innovation, Enterpreneurship and Technology, 103– 108.

Widiputri, D.I., Mariana, N., Josopandojo, B., Gunawan-Puteri, M.D.P.T., Kartawiria, I.S., 2017. Effect of Pre-treatment Processes and Stability Testing of Lemongrass (Cymbopogon citratus) Extract on α-Glucosidase Inhibitor (AGI) and α-Amylase Inhibitor (AAI) activities. Proceedings of International Postgraduate Symposium on Food, Agriculture, and Biotechnology, pp. 10-20.

Yu, Y., Zick, S., Li, X., Zou, P., Wright, B., Sun, D., 2011. Examination of the Pharmacokinetics of Active Ingredients of Ginger in Humans. The AAPS Journal, 13(3), 417–426.



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