CHOLESTEROL LOWERING EFFECT OF CHITOSAN NANOPARTICLES  USING PARIJOTO FRUITS EXTRACT

Fania Putri Luhurningtyas; Rissa Laila Vifta; Nur Syarohmawati; Mahardika Adhi Candra

doi:10.24071/jpsc.002017

CHOLESTEROL LOWERING EFFECT OF CHITOSAN NANOPARTICLES USING PARIJOTO FRUITS EXTRACT

Fania Putri Luhurningtyas^(1*), Rissa Laila Vifta⁽²⁾, Nur Syarohmawati⁽³⁾, Mahardika Adhi Candra⁽⁴⁾,

(1) Program Studi S1 Farmasi, Universitas Ngudi Waluyo
(2) Program Studi S1 Farmasi, Universitas Ngudi Waluyo
(3) Program Studi S1 Farmasi, Universitas Ngudi Waluyo
(4) Program Studi S1 Farmasi, Universitas Ngudi Waluyo
(*) Corresponding Author

Abstract

Parijoto (Medinilla speciosa Reinw. ex Blum) fruit is known to have pharmacological activity as cholesterol lowering levels. Its activity needs to be increased with nanoparticle system so that the active substance can bind 100% to the action target. This study aims to determine the formation of nanoparticles from parijoto fruit (NEBP) and activity test as a decrease in cholesterol levels. The formation of nanoparticles used variations of concentration and volume of chitosan and NaTPP. Anti-cholesterol testing is based on the amount of free cholesterol in the sample that reacted with Lieberman-Burchard into complex green compounds. The best formation of NEBP was 0.2% chitosan, 0.1% NaTPP and volume ratio 5:1. The particle size showed an average size of 269.3 nm (10-1000 nm). The result of the percent transmittance and polydispersity index were 99,379 (close to 100%) and 0.378 (PDI <0.5). The functional group-specific of NEBP was OH, N-H, PO₃. The morphology was round and non-uniform particles. NEBP can decrease 50% cholesterol levels with a smaller EC₅₀ value was 89.08 compared to the extract (EC₅₀ 259.98 ppm). Nanoparticles of parijoto fruit is a potential candidate for anti-cholesterol drug.

Keywords

anti-cholesterol; fruit; Medinilla speciosa Reinw. ex Blum; nanoparticles.

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References

Anggraini, D.I., Nabillah, L.F. 2018. Activity test of suji leaf extract (dracaena angustifolia roxb.) on in vitro cholesterol lowering. Jurnal Kimia Sains dan Aplikasi, 21(2), 54-58.

Anggraini, D.I., Lily Fathrah N. 2017. Activity test of suji leaf extract (dracaena angustifolia roxb.) on in vitro cholesterol lowering. Journal of Scientific and Applied Chemistry, 21(2), 54-58.

Bastien, M., Poirier, P., Lemieux, I., Desprs, J.P. 2014. Overview of epidemiology and contribution of obesity to cardiovascular disease. Progress in cardiovascular diseases, 56, (4), 369-381

Bhosale, A.P., Patil, A., Swami, M. 2015. Herbosomes as a novel drug delivery system for absorption enhancement. World Journal of Pharmacy and Pharmaceutical Sciences, 5(1), 345-355.

Bunglavan, S.J., Garg, A.K., Dass, R.S., Shrivastava, S. 2014. Use of nanoparticles as feed additives to improve digestion and absorption in livestock. Livest. Res. Int, 2, 36-47.

Dewandari, K.T., Yuliani, S., Sedamawati, S. 2013. Ekstraksi, karakterisasi nanopartikel ekstrak sirih merah (piper crocatum). Jurnal Pascapanen, 10 (2), 58-65.

Elgadir, M.A., Uddin, M.S., Ferdosh, S., Adam, A., Chowdhury, A.J.K., Sarker, M.Z.I. 2015. Impact of chitosan composites and chitosan nanoparticle composites on various drug delivery systems: A review. Journal of food and drug analysis, 23(4), 619-629.

Fathinatullabibah, F., Khasanah, L.U., Kawiji, K. 2014. Stabilitas antosianin ekstrak daun jati (Tectona grandis) terhadap perlakuan pH, suhu. Jurnal Aplikasi Teknologi Pangan, 3(2), 60-63.

Ferreira-Tomaz, A., Sobral de Carvalho, S., Barbosa, C.R., L Silva, S., Sabino Gutierrez, M., B de Lima, A., L Fook, M. 2018. Ionically crosslinked chitosan membranes used as drug carriers for cancer therapy applications. Materials. 11(10), 2051.

Irawati, T., Mardiana, Y. 2018. Stabilitas antosianin dari ekstrak buah mangsi (phyllanthus reticulatus poir). Jurnal Ilmiah Hijau Cendekia, 3(2), 26-29.

Kleine-brueggeney, H., Zorzi, G.K., Fecker, T., El Gueddari, N.E., Moerschbacher, B.M., Goycoolea, F.M. 2015. A Rational approach towards the design of chitosan-based nanoparticles obtained by ionotropic gelation. Colloids and Surfaces B: Biointerfaces, 135, 99-108.

Lusiana, R.A., Wahyu P.P. 2018. Membran kitosan termodifikasi tripolifosfat-heparin, aplikasinya pada permeasi urea, kreatinin. Analytical and Enviromental Chemistry, 3(1), 11-21.

Mardliyati, E., Muttaqien, S.E., Setyawati, D. R. 2012. Sintesis nanopartikel kitosan-trypoly phosphate dengan metode gelasi ionik: pengaruh konsentrasi, rasio volume terhadap karakteristik partikel. Prosiding Pertemuan Ilmiah Ilmu Pengetahuan, Teknologi Bahan.

Mohammed, M., Syeda, J., Wasan, K., Wasan, E. 2017. An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics, 9(4), 53.

Najib, A., Malik, A., Ahmad, A.R., Handayani, V., Syarif, R.A., Waris, R. 2017. Standarisasi ekstrak air daun jati belanda, teh hijau. Jurnal Fitofarmaka Indonesia, 4(2), 241245.

Napsah, R., Wahyuningsih, I. 2014. Preparasi nanopartikel kitosan-tpp/ekstrak etanol daging buah mahkota dewa (phaleriamacrocarpa (scheff) boerl) dengan metode gelasi ionik. Jurnal Farmasi Sains dan Komunitas, 11(1), 7-12.

Ningsih, N., Sedarnawati Y., Sri Y. 2017. Sintesis nanopartikel ekstrak kulit manggis merah, kajian sifat fungsional produk enkapsulasinya. Jurnal Teknologi, Industri Pangan, 28(1), 27-35.

Republik Indonesia, Kementerian Kesehatan. 2018. Hasil utama riskesdas 2018. Jakarta: Badan Penelitian, Pengembangan Kesehatan.

Rizvi, S.A., Saleh, A.M. 2018. Applications of nanoparticle systems in drug delivery technology. Saudi Pharmaceutical Journal, 26(1), 64-70.

Saadah, N.N., Purwani, K.I., Nurhayati, A. P.D., Ashuri, N.M. 2017. Analysis of lipid profile and atherogenic index in hyperlipidemic rat (rattus norvegicus berkenhout, 1769) that given the methanolic extract of parijoto (medinilla speciosa). AIP Conference Proceedings, 1854(1), AIP Publishing.

Shah, B.R., Li, Y., Jin, W., An, Y., He, L., Li, Z., Li, B. 2016. Preparation and optimization of Pickering emulsion stabilized by chitosan-tripolyphosphate nanoparticles for curcumin encapsulation. Food Hydrocolloids, 52, 369-377.

Sugiarti, L., Pujiastuti, E. 2017. Uji aktivitas antibakteri ektrak etanol buah parijoto (medinilla speciosa blume) terhadap bakteri staphylococcus aureus, echerichia coli. Cendekia Journal of Pharmacy, 1(1), 25-33.

Sulistyawati, R., Nurani, L.H., Hidayati, S., Mursyidi, A., Mustofa, M. Standarisasi kualitas fraksi etil asetat daun kelor (moringa oleifera lamk.). URECOL. 67-72.

Suptijah, P., Jacoeb, A.M., Rachmania, D. 2011. Karakterisasi nano kitosan cangkang udang vannamei (litopenaeus vannamei) dengan metode gelasi ionik. Jurnal Pengolahan Hasil Perikanan Indonesia, 14(2), 78-84.

Vifta, R., Advistasari, Y.D. 2018. Analisis penurunan kadar glukosa fraksi n-heksan buah parijoto (medinilla speciosa b) secara in vitro dengan metode spektrofotometri uv-vis. Indonesian Journal of Chemical Science, 7(3), 249-253.

Wachidah, L.N. 2013. Uji aktivitas antioksidan, serta penentuan kandungan fenolat, flavonoid total dari buah parijoto (medinilla speciosa blume). Fakultas Kedokteran, Ilmu Kesehatan UIN Syarif Hidayatullah, Jakarta.

DOI: https://doi.org/10.24071/jpsc.002017