UJI AKTIVITAS ANALGESIK ASAM 2-(3-(KLOROMETIL)BENZOILOKSI)BENZOAT DAN ASAM 2-(4-(KLOROMETIL)BENZOILOKSI)BENZOAT PADA TIKUS WISTAR JANTAN DENGAN METODE PLANTAR TEST

Wahyu Dewi Tamayanti, Ratna Megawati Widharna, Caroline Caroline, Bambang Soekarjo

Abstract


The new compounds of salicylic acid derivatives, 2- (3- (chloromethyl) benzoyloxy) benzoic acid and 2- (4- (chloromethyl) benzoyloxy) benzoic acid were synthesized to generate better analgesic activity and lesser stomach irritation of salicylic acid. The 2- (4- (chloromethyl) benzoyloxy) benzoic acid and 2- (3- (chloromethyl) benzoiloksi) benzoic acid were synthesized by Schotten-Baumann acylation process. In this study, analgesic activity was tested by plantar test method. The compounds were administered to the tested animals at 12.5; 25; 50; 100; and 200 mg / kg BW of doses. Analgesic activity was determined by the response time of rat to pain induced by infra red. The percentage of pain hindrance was calculated from the mean of response time to pain. Pain hindrance percentage were shown as: 74.28%; 105.58%; 110.58%; 115.29%; and 175.87% after administration of 2- (4- (chloromethyl) benzoyloxy) benzoic acid at doses of 12.5; 25; 50; 100; and 200 mg / kg. The pain hindrance percentage after administration of 2- (3- (chloromethyl) benzoyloxy) benzoaic acid, were 85.30%; 92.48%; 124.96%; 180.36%; and 208.01% respective to the doses of 12.5; 25; 50; 100; and 200 mg / kg. Five doses of acetyl salicylic acid ranged from 12.5 mg / kg to 200 mg / kg showed pain hindrance percentage as: 26%; 34.34%; 45.68%; 60.38%; and 114.12%. This study indicated that 2- (3- (chloromethyl) benzoyloxy) benzoic acid and 2- (4- (chloromethyl) benzoyloxy) benzoic acid generated higher analgesic activity than acetyl salicylic acid.


Keywords


analgesic activity; plantar test; 2-(3-(chloromethyl)benzoyloxy)benzoic acid; 2-(4-(chloromethyl)benzoyloxy)benzoic acid

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References


Furst, D.E., & Munster. T., 2000. Obat-obat Antiinflamasi Nonsteroid, Obat-obat Antireumatik Pemodifikasi-penyakit, Analgesik Nonopioid dan Obat-obat Untuk Pirai, in Katzung B.G. (Ed.), Farmakologi Dasar dan Klinik: Basic dan Clinical Pharmacologi, 8th ed, Salemba Medika, Jakarta, 455, 459-461.

Gunawan S.G., 2009. Farmakologi dan Terapi Edisi 5, Bagian Fakultas Kedokteran Universitas Indonesia, Jakarta, 231-233, 235.

Martak R., Soekardjo, B., Surdijati, S., Caroline and Setyabudi, I., 2009. Synthesis of 4-Chloromethylbenzoyl Salicylic Acid and Its Analgesic Activity on Mice (Mus musculus), Poster Presentation in Bandung International Conference on Medicinal Chemistry, School of Pharmacy, Bandung.

McMurry, J., & Simanek, E., 2007. Fundamentals of Organic Chemistry 6th ed., Examview and Examview pro are Registered Trademarks of FsCreations, Inc., America, 413-417.

Mishra, 2011. Analgesic and Inflammatory Activity of Methanol Extract of Scindapsus officinalis Root in Experimental Animals, Odisha, India.

Natalia, O. Caroline C., Soekardjo B., 2013. Pemodelan Interaksi Turunan Potensial Asam Benzoil Salisilat dengan Reseptor Enzim Siklooksigenase-2, Jurnal Farmasi Sains dan Terapan, 1(1), 19-24.

Pratiwi, D.V., 2009. Sintesis Asam 3-klorometilbenzoil Salisilat dan Uji Aktivitas Analgesik pada Mencit (Mus musculus), Skripsi Sarjana Farmasi, Universitas Katolik Widya Mandala Surabaya.

Purwanto & Susilowati, R., 2000. Hubungan struktur-aktivitas obat analgetika, In: Siswandono dan Soekardjo, B., (Eds.), Kimia Medisinal 2, Airlangga University Press, Surabaya, 283, 291-292, 295.

Robert, M.S., Francis, X.W., and David, J.K., 2005. Spectrofotometric Identification of Organic Compounds 7th ed., John Wiley and Sons, Inc. America, 72-101.

Siswandono & Susilowati, R., 2000. Hubungan kuantitatif struktur-aktivitas, in Siswandono dan Soekardjo, B., (Eds.), Kimia Medisinal 1, Airlangga University Press, Surabaya, 265, 304.

Soekardjo B., Caroline, Widharna R.M., 2011. Uji Toksisitas Akut Penentuan LD50 Senyawa Turunan Asam Benzoilsalisilat pada Mencit, LPPM Research Project, Unika Widya Mandala, Surabaya.

Vogel, H.G, 2008. Drug Discovery and Evaluation Pharmacological Assay, 3rd ed. Springer-verlag, Berlin, 1010-1014, 1031.




DOI: https://doi.org/10.24071/jpsc.00125

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