ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI

Agustina Setiawati(1), Sri Hartati Yuliani(2*), Enade Perdana Istyastono(3), Michael Raharja Gani(4), Evy Fenny Veronica(5), Dina Christin Ayuning Putri(6), Reza Eka Putra(7), David Chandra Putra(8), Agnes Mutiara Kurniawan(9),

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(*) Corresponding Author

Abstract


Rapid and simple quantitative analysis isoflavones tempe using densitometric TLChas been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5:0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distanceof 1 ?L sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linearrelationship obtained at 0.08 - 2 ?g/spot with r= 0.9986. The LOD and LOQ of isoflavone were0.014 ?g/spot and 0.048 ?g/spot. Genistein contained in tempe was 0.151 0.005 % b/b.

Keywords


tempe; isoflavon; TLC

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DOI: https://doi.org/10.24071/jpsc.0080

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Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community)

Published by Faculty of Pharmacy, Universitas Sanata Dharma Yogyakarta

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