ANTIOXIDANT ACTIVITY AND ANTIAGING OF ETHANOLIC EXTRACT OF RED BEETROOT (BETA VULGARIS L.) IN VITRO

Aji Winanta

doi:10.24071/jpsc.004722

ANTIOXIDANT ACTIVITY AND ANTIAGING OF ETHANOLIC EXTRACT OF RED BEETROOT (BETA VULGARIS L.) IN VITRO

Aji Winanta^(1*),

(1) Universitas Muhammadiyah Yogyakarta
(*) Corresponding Author

Abstract

Aging is a process of gradual changes in the skin caused by intrinsic and extrinsic factors. Intrinsic and extrinsic aging is the effect of increasing the reactive oxygen species (ROS) produced by cells resulting in cell damage. The use of natural materials expanded for skin damage therapy, one of which is the utilization of red beetroot (Beta vulgaris L.). This study aims to discover the antioxidant activity and antiaging activity in vitro. The process begins with the extraction of the maceration method using 70% ethanol as a solvent. Further identification of secondary metabolite compounds by phytochemical screening. Antioxidant activity test using DPPH method with quercetin as a comparison. Antiaging activity test in vitro with cell viability test using Human Dermal Fibroblast adult (HDF-a) by MTT Assay and the inhibitory effect of skin degradation enzymes (antiaging) was carried out with elastase using Human Neutrophil Elastase (HNE). The result of phytochemical screening tests showed red beetroot extract (Beta vulgaris L.) contained alkaloid, saponin, and triterpenoid compounds. Red beetroot extract has weak antioxidant activity with IC50 of 3284.42 μg/mL. Antiaging activity testing showed that red beetroot extract can maintain cell viability and has the inhibitory activity of the enzyme elastase. This study concludes that red beetroot extract (Beta vulgaris L.) is potential as a cosmetic active ingredient for antiaging

Keywords

beetroot; anti-aging; cell viability; inhibition of elastase enzymes; in vitro

References

Ahmad, Z. (2018). Penuaan Kulit: Patofisiologi dan Manifestasi Klinis. 30(3), 8.

Alasa, A. N., Anam, S., & Jamaluddin, J. (2017). Analisis Kadar Total Metabolit Sekunder Ekstrak Etanol Daun Tamoenju (Hibiscus surattensis L.). Kovalen, 3(3), 258. https://doi.org/10.22487/j24775398.2017.v3.i3.9334

Asra, R., Yetti, R. D., Ratnasari, D., & Nessa, N. (2020). Studi Fisikokimia Betasianin Dan Aktivitas Antioksidan Dari Umbi Bit Merah (Beta Vulgaris L.). Journal of Pharmaceutical And Sciences, 3(1), 14–21. https://doi.org/10.36490/journal-jps.com.v3i1.35

Elisabeth, et all. (2020). Identifikasi Metabolit Sekunder dan Uji Aktivitas Antioksidan Ekstrak Etanol Umbi Bit Merah (Beta vulagris l.) dengan Metode DPPH. 3, 13.

Girsang, E., Lister, I. N. E., Ginting, C. N., Sholihah, I. A., Raif, M. A., Kunardi, S., Million, H., & Widowati, W. (2020). Antioxidant and antiaging activity of rutin and caffeic acid. Pharmaciana, 10(2), 147. https://doi.org/10.12928/pharmaciana.v10i2.13010

Harborne, J. B. (1996). Metode Fitokimia Penuntun Cara Modern Menganalisa Tumbuhan (2nd ed.). ITB Bandung.

Jia, N., Li, T., Diao, X., & Kong, B. (2014). Protective effects of black currant (Ribes nigrum L.) extract on hydrogen peroxide-induced damage in lung fibroblast MRC-5 cells in relation to the antioxidant activity. Journal of Functional Foods, 11, 142–151. https://doi.org/10.1016/j.jff.2014.09.011

Juliana, C., Lister, I. N. E., Girsang, E., Nasution, A. N., & Widowati, W. (2020). Antioxidant and Elastase Inhibitor from Black Soybean (Glycine max L.) and Its Compound (Daidzein). Journal of Biomedicine and Translational Research, 6(1), 11–14. https://doi.org/10.14710/jbtr.v6i1.5540

Karim, A. A., Azlan, A., Ismail, A., Hashim, P., Abd Gani, S. S., Zainudin, B. H., & Abdullah, N. A. (2014). Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract. BMC Complementary and Alternative Medicine, 14(1), 381. https://doi.org/10.1186/1472-6882-14-381

Kim, D.-B., Shin, G.-H., Kim, J.-M., Kim, Y.-H., Lee, J.-H., Lee, J. S., Song, H.-J., Choe, S. Y., Park, I.-J., Cho, J.-H., & Lee, O.-H. (2016). Antioxidant and anti-ageing activities of citrus-based juice mixture. Food Chemistry, 194, 920–927. https://doi.org/10.1016/j.foodchem.2015.08.094

Nur, S. (2017). Pemanfaatan Ekstrak Etanolik Dan Etil Asetat Daging Buah Dan Kulit Buah Langsat (Lansium Domesticum (Corr)) Sebagai Bahan Aktif Kosmetik: Uji Aktivitas Antiaging Dan Anti Jerawat Secara In Vitro. Thesis (Universitas Gadjah Mada), 208.

Nur, S., Rumiyati, R., & Lukitaningsih, E. (2017). Screening Of Antioxidants, Anti-Aging And Tyrosinase Inhibitory Activities Of Ethanolic And Ethyl Acetate Extracts Of Fruit Flesh And Fruit Peel Langsat (Lansium Domesticum Corr) In Vitro. Majalah Obat Tradisional, 22(1), 63. https://doi.org/10.22146/tradmedj.24342

Patria, W.D., Soegihardjo, C.J. (2013). Uji Aktivitas Antioksidan Menggunakan Radikal 1,1-Difenil-2-Pikrilhidrazil (Dpph) Dan Penetapan Kandungan Fenolik Total Fraksi Etilasetat Ekstrak Etanolik Daun Benalu (Dendrophthoe pentandra L. Miq.) Yang Tumbuh Di Pohon Kepel (Stelechocarpus burahol (Bl.) Hook. f.). J. Farm. Sains Dan Komunitas 10, 52–60. https://doi.org/10.24071/jpsc.0090

Pouillot, A., Polla, L. L., Tacchini, P., Neequaye, A., Polla, A., & Polla, B. (2011). Natural Antioxidants and their Effects on the Skin. In N. Dayan & L. Kromidas (Eds.), Formulating, Packaging, and Marketing of Natural Cosmetic Products (pp. 239–257). John Wiley & Sons, Inc. https://doi.org/10.1002/9781118056806.ch13

Pratiwi, N. F., Kristiani, E. B. E., & Kasmiyati, S. (2019). Buah Naga Berpotensi Lebih Tinggi sebagai Sumber Antioksidan Dibandingkan Buah Bit. 5.

Sutjiatmo, A. B., Edriayani, N., Mulyasari, T. E., Hermanto, F., Fahrauk, M., Sukandar, E. Y., Kusuma, H. S. W., Rizal, R., & Widowati, W. (2020). Antioxidant and Antiaging Assays of Ageratum conyzoides (L.) Ethanolic Extract. Pharmaceutical Sciences and Research, 7(3). https://doi.org/10.7454/psr.v7i3.1061

Widyawati, P. S., Wijaya, C. H., Harjosworo, P. S., & Sajuthi, D. (2010). Pengaruh Ekstraksi Dan Fraksinasi Terhadap Kemampuan Menangkap Radikal Bebas Dpph (1,1-Difenil-2- Pikrilhidrazil) Ekstrak Dan Fraksi Daun Beluntas. 7.

DOI: https://doi.org/10.24071/jpsc.004722