Identification Bioactive Compound of Ethanol-Water Fraction of Coleus atropurpureus for Anti-rheumatic Rheumatism in CFA-induced Rats

Ipang Djunarko; Nanang Fakhrudin; Arief Nurrochmad; Subagus Wahyuono

doi:10.24071/jpsc.004746

Identification Bioactive Compound of Ethanol-Water Fraction of Coleus atropurpureus for Anti-rheumatic Rheumatism in CFA-induced Rats

Ipang Djunarko⁽¹⁾, Nanang Fakhrudin^(2*), Arief Nurrochmad⁽³⁾, Subagus Wahyuono⁽⁴⁾,

(1) Postgraduate Program of Pharmacy, Faculty of Pharmacy, Gadjah Mada University
(2) Department of Pharmaceutical Biology, Faculty of Pharmacy, Gadjah Mada University
(3) Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Gadjah Mada University
(4) Department of Pharmaceutical Biology, Faculty of Pharmacy, Gadjah Mada University
(*) Corresponding Author

Abstract

Nonsteroidal anti-inflammatory drugs are used for pain and to slow the progression of rheumatoid arthritis. Accordingly, the discovery of rheumatoid arthritis active compounds from the ethanol-water fraction Coleus atropurpureus (EWC) was conducted to characterize the isolated compounds as well as the anti-rheumatic effects of the EWC induced Complete Freund's Adjuvant (CFA). We conducted in vivo study in rats which were randomly divided into 5 groups. Group 1 was only given CFA as a negative control. Group 2 as positive control was orally exposed to diclofenac potassium (9 mg/BW). Three groups were given different EWCs orally as follows: 11.25 mg/BW, 22.5 mg/BW, and 45 mg/BW, respectively. Rheumatism rates were then compared with positive controls using a visual arthritic scoring system. The compounds identified by isolation of the EWC of Coleus atropurpureus predicted forskolin. The ethanol-water fraction Coleus atropurpureus did not act as an anti-rheumatic arthritis agent in CFA-induced rats.

Keywords

Anti-rheumatic arthritis; Coleus atropurpureus; Complete Freund's Adjuvant

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DOI: https://doi.org/10.24071/jpsc.004746