Characterization of 1H NMR and 13C NMR Spectrometry and Antibacterial Activities of 4-tert-Butylbenzoyl Amoxicillin

Hadi Barru Hakam Fajar Siddiq; Dewi Rashati; Siti Nur Azizah

doi:10.24071/jpsc.002183

Characterization of 1H NMR and 13C NMR Spectrometry and Antibacterial Activities of 4-tert-Butylbenzoyl Amoxicillin

Hadi Barru Hakam Fajar Siddiq^(1*), Dewi Rashati⁽²⁾, Siti Nur Azizah⁽³⁾,

(1) Department of Pharmacy, Akademi Farmasi Jember, Jember, Indonesia
(2) Department of Pharmacy, Akademi Farmasi Jember, Jember, Indonesia
(3) Department of Pharmacy, Akademi Farmasi Jember, Jember, Indonesia
(*) Corresponding Author

Abstract

The synthesis of 4-tert-butylbenzoyl amoxicillin has been done by reacting amoxicillin with 4-tert-butylbenzoyl chloride. The product was characterized using proton and carbon-13 spectrometry. Antibacterial activity of 4-tert-butylbenzoyl amoxicillin against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus was tested using paper disk diffusion method. Results showed that 4-tert-butylbenzoyl amoxicillin had a specific spectrum activity. Antibacterial test of 4-tert-butylbenzoyl amoxicillin showed clear zones around the disc paper for Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The study results indicated 4-tert-butylbenzoyl amoxicillin has antimicrobial properties but is categorized as a less-effective antibiotic agent based on the response to bacterial growth inhibition.

Keywords

4-tert-butylbenzoyl amoxicillin; Bacillus subtilis; Escherichia coli; spectrometry; Staphylococcus aureus

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DOI: https://doi.org/10.24071/jpsc.002183