Yunita Linawati


Hepatoprotective effect study of forest honey had been conducted on a female rat induced with carbon tetrachloride (CCl4). The study aimed at obtaining the scientific data and the evidence of forest honey as hepatoprotective agent on the rat. The study was a true experimental study with a single factor completely randomized design. Thirty rats were randomly divided into six groups (n=5). Group I received carbon tetrachloride 2.0 mL/kgBW intraperitoneally, group II received olive oil 2.0 mL/kgBW intraperitoneally, group III received forest honey 8.1 mL/kgBW (6 days, peroral), groups IV, V, VI were given forest honey 3.6, 5.4, 8.1 mL/kgBW (6 days, peroral) and intraperitoneal induction of carbon tetrachloride 2 mL/kgBW on seventh day. The blood sample of all rats were taken for ALT-AST measurement and their liver were sampled for histological examination of the liver cell. Groups I and III on the seventh day, group II on the second day, groups IV,V,VI on the eighth day. The result showed that a forest honey can be used as a hepatoprotective agent on the female rat Wistar strain induced by carbon tetrachloride 2 mL/kgBW with doses 3.6, 5.4, 8.1 mL/kgBW.


ALT-AST; carbon tetrachloride; forest honey; liver histopathology

Full Text:



Chen, L., Mehta, A., Barenbaum, M., Zanger, A.R., and Engeseth, N.J., 2000. Honeys from Different Floral Sources as Inhibitors of Enzymatic Browning in Fruit and Vegetable Homogenates, Journal of Agricultural and Food Chemistry, 48(10), 4997– 5000.

Erguder, B.I., Kilicoglu, S.S., Namuslu, M., Kilicoglu, B., Devrim, E., Kismet, K., and Durak, I., 2008. Honey prevent hepatic damage induced by obstruction of the common bile duct, World Journal of Gastroenterology, 12 (23), 3729-3732.

Halawa, H.M., El-Nefiawy, N.E., Makhloul, N.A., and Mady, A.A., 2009. Evaluation of Honey Protective on Lead Induced Oxidatives Stress in Rats, Journal of the Arab Society for Medical Research, 4 (2), 197 – 209.

Jamila, N., Khan, N., Khan, A.A., Khan, I., Khan, S.N., Zakaria, Z.A., Khairuddean, M., Osman, H., and Kim, K.S., 2017. In Vivo Carbon Tetrachloride-Induced Hepatoprotective and In Vivo Cytotoxic Activities of Garcinia hombroniana (Seashore Mangosteen). African Journal of Traditional, Complimentary & Alternative Medicines, 14 (2), 374-382.

Kandimalla, R., Kalita, S., Saikia, B.,Choudhury, B., Singh, Y., Kalita, K., Dash, S., and Kotoky, J., 2016. Antioxidant and Hepatoprotective Potentiality of Randia dumetorum Lam. Leaf and Bark via Inhibition of Oxidative Stress and Inflammatory Cytokines. Journal Frontiers in Pharmacology, 7 (205), 1-8.

Mahesh, A., Shaheetha, J., Thangadurai, D., and Rao, D.M., 2009. Protective Effect of Indian Honey on Acetaminophen Induced Oxidative Stress and Liver Toxicity in Rat, Journal Biologia, 64 (6), 1225 – 1231.

Monika, B., 2012. Propolis prevents hepatorenal injury induced by chronic exposure to carbon tetrachloride, hindawi publishing corporation, Evidence Based Complementary and Alternative Medicine, 2012, ID235358.

Nagai, T., Inoue, R., Kanamori, N., Suzuki, N., and Nagashima, T., 2006. Characterization of Honey from Different Floral Sources. Its Functional Properties and Effects of Honey Species on Storage of Meat, Food Chemistry, 97, 256 – 262.

Price, S.A., 2005. Patofisiologi konsep klinis proses-proses penyakit. Volume 1. Jakarta: EGC.

Sarin, S.K., Kedarisetty, C.K., Abbas, Z., Amarapurkar, D., Bihari, C., Chan, A.C., et al., 2009. Acute on-chronic liver failure: consensus recommendations of the Asian Pacific Association for The Study of The Liver (APASL) 2014. Hepatology International, 8(4), 453-471.

Suranto, A., 2007. Terapi Lebah, Penebar Plus, Jakarta.

Thapa, B.R., and Anuj, W., 2007. Liver Function Tests and their Interpretation. Indian Journal of Pediatrics, 74, 67-75.

Tjok, I.A.S., and Wibawa, I.D.N., 2012. Pendekatan diagnosis dan terapi fibrosis hati., Denpasar: Bagian / SMF Ilmu Penyakit Dalam FK Universitas Udayana / RS Sanglah.

Utomo, L.S., 2015. Efek Hepatoprotektif Pemberian Jangka Panjang Infusa Herba Bidens pilosa L. terhadap Aktivitas ALT-AST Serum pada Tikus Betina Terinduksi Karbon Tetraklorida. Skripsi. Fakultas Farmasi Universitas Sanata Dharma, Yogyakarta.

DOI: http://dx.doi.org/10.24071/jpsc.151983


  • There are currently no refbacks.

Copyright (c) 2018 Journal of Pharmaceutical Sciences and Community










Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community)

Published by Faculty of Pharmacy, Universitas Sanata Dharma Yogyakarta

Creative Commons Licence
This work is licensed under a Creative Commons Attribution 4.0 International License.

Web Analytics JPSC Stats